Project name: SH3_Y132T

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA132T
Energy difference between WT (input) and mutated protein (by FoldX)	2.6645 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1831
• Maximal score value: 1.7964
• Average score: -1.0334
• Total score value: -58.9045

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5183
86	L	A	0.7945
87	F	A	0.9243
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6568
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1323
99	D	A	-3.1831
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2369
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6420
109	F	A	0.0000
110	Q	A	-0.5834
111	I	A	-0.2277
112	L	A	-0.0546
113	N	A	-1.1038
114	S	A	-1.3412
115	S	A	-1.6695
116	E	A	-2.6594
117	G	A	-2.2375
118	D	A	-2.5736
119	W	A	-1.3650
120	W	A	-1.4573
121	E	A	-1.8831
122	A	A	0.0000
123	R	A	-1.8761
124	S	A	0.0000
125	L	A	0.0591
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3579
129	E	A	-2.2417
130	T	A	-1.8845
131	G	A	-1.8349
132	T	A	-1.3738	mutated: YA132T
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-1.0522
136	N	A	-1.1583
137	Y	A	-0.1302
138	V	A	0.0000
139	A	A	0.4149
140	P	A	0.7757
141	V	A	1.7964