Project name: SH3_L86I

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA86I
Energy difference between WT (input) and mutated protein (by FoldX)	2.07413 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8097
• Average score: -0.926
• Total score value: -52.783

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5554
86	I	A	0.8928	mutated: LA86I
87	F	A	0.9695
88	V	A	0.4462
89	A	A	0.0000
90	L	A	-0.1231
91	Y	A	-0.5390
92	D	A	-2.5221
93	Y	A	-1.9321
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.6820
105	K	A	-2.3112
106	G	A	-1.2547
107	E	A	-1.1783
108	K	A	-0.5576
109	F	A	0.0000
110	Q	A	-0.4088
111	I	A	-0.0370
112	L	A	0.1480
113	N	A	-0.8856
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0536
121	E	A	-1.1503
122	A	A	0.0000
123	R	A	-1.6195
124	S	A	0.0000
125	L	A	0.4067
126	T	A	-0.2819
127	T	A	-0.7225
128	G	A	-1.2425
129	E	A	-2.1808
130	T	A	-1.6495
131	G	A	-1.4992
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1511
137	Y	A	-0.1394
138	V	A	0.0000
139	A	A	0.4149
140	P	A	0.7694
141	V	A	1.8097