Project name: SH3_V141G

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA141G
Energy difference between WT (input) and mutated protein (by FoldX)	1.99114 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 0.235
• Average score: -1.0813
• Total score value: -61.6357

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.0941
86	L	A	0.2350
87	F	A	0.1791
88	V	A	-0.2670
89	A	A	0.0000
90	L	A	-0.1749
91	Y	A	-0.5818
92	D	A	-2.5594
93	Y	A	-1.9312
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7465
105	K	A	-2.4303
106	G	A	-1.7263
107	E	A	-1.3550
108	K	A	-1.0670
109	F	A	0.0000
110	Q	A	-0.4774
111	I	A	-0.0387
112	L	A	0.1378
113	N	A	-0.8903
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0578
121	E	A	-1.1558
122	A	A	0.0000
123	R	A	-1.6968
124	S	A	0.0000
125	L	A	0.1242
126	T	A	-0.4512
127	T	A	-0.8056
128	G	A	-1.3338
129	E	A	-2.2265
130	T	A	-1.6857
131	G	A	-1.4991
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1501
137	Y	A	-0.1338
138	V	A	0.0000
139	A	A	-0.1677
140	P	A	-0.2999
141	G	A	-0.4225	mutated: VA141G