Project name: SH3_L125R

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA125R
Energy difference between WT (input) and mutated protein (by FoldX)	1.13459 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7452
• Average score: -1.1683
• Total score value: -66.5904

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4308
86	L	A	0.2432
87	F	A	0.5247
88	V	A	0.0632
89	A	A	0.0000
90	L	A	-0.1520
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7292
105	K	A	-2.4071
106	G	A	-1.4602
107	E	A	-1.9268
108	K	A	-1.6556
109	F	A	0.0000
110	Q	A	-1.2411
111	I	A	-0.1182
112	L	A	0.1284
113	N	A	-0.8924
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0622
121	E	A	-1.1632
122	A	A	0.0000
123	R	A	-2.5167
124	S	A	0.0000
125	R	A	-2.9368	mutated: LA125R
126	T	A	-1.9495
127	T	A	-1.6819
128	G	A	-2.2690
129	E	A	-2.7224
130	T	A	-2.0343
131	G	A	-1.5047
132	Y	A	-0.8707
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.3909
140	P	A	0.7246
141	V	A	1.7452