Project name: SH3_V88V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA88V
Energy difference between WT (input) and mutated protein (by FoldX)	-0.00669988 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7945
• Average score: -0.9606
• Total score value: -54.7519

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5171
86	L	A	0.7907
87	F	A	0.9205
88	V	A	0.4264	mutated: VA88V
89	A	A	0.0000
90	L	A	-0.1500
91	Y	A	-0.5739
92	D	A	-2.5594
93	Y	A	-1.9312
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7290
105	K	A	-2.4066
106	G	A	-1.4602
107	E	A	-1.3121
108	K	A	-0.6515
109	F	A	0.0000
110	Q	A	-0.5111
111	I	A	-0.0577
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7167
124	S	A	0.0000
125	L	A	0.0563
126	T	A	-0.4748
127	T	A	-0.8215
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.4992
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1501
137	Y	A	-0.1239
138	V	A	0.0000
139	A	A	0.4148
140	P	A	0.7742
141	V	A	1.7945