| Chain sequence(s) |
A: RAFRGPLLPNRPFTTVWNANTQWCLERHGVDVDVSVFDVVANPGQTFRGPDMTIFYSSQLGTYPYYTPTGEPVFGGLPQNASLIAHLARTFQDILAAIPAPDFSGLAVIDWEAWRPRWAFNWDTKDIYRQRSRALVQAQHPDWPAPQVEAVAQDQFQGAARAWMAGTLQLGRALRPRGLWGFYGFPDCYNYDFLSPNYTGQCPSGIRAQNDQLGWLWGQSRALYPSIYMPAVLEGTGKSQMYVQHRVAEAFRVAVAAGDPNLPVLPYVQIFYDTTNHFLPLDELEHSLGESAAQGAAGVVLWVSWENTRTKESCQAIKEYMDTTLGPFILNVTSGALLCSQALCSGHGRCVRRTSHPKALLLLNPASFSIQLTPGGGPLSLRGALSLEDQAQMAVEFKCRCYPGWQAPWCERKSMWT |
| Distance of aggregation | 10 Å |
| Dynamic mode | No |
| Mutated residues | FA22A |
| Energy difference between WT (input) and mutated protein (by FoldX) | 0.47861 kcal/mol
Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive. |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 20 | R | A | -2.1579 | |
| 21 | A | A | -1.4907 | |
| 22 | A | A | -0.8234 | mutated: FA22A |
| 23 | R | A | -1.0382 | |
| 24 | G | A | -0.6629 | |
| 25 | P | A | -0.7172 | |
| 26 | L | A | 0.0000 | |
| 27 | L | A | -0.5639 | |
| 28 | P | A | -0.9195 | |
| 29 | N | A | -1.7169 | |
| 30 | R | A | -1.1604 | |
| 31 | P | A | 0.0000 | |
| 32 | F | A | 0.0000 | |
| 33 | T | A | 0.0000 | |
| 34 | T | A | 0.0000 | |
| 35 | V | A | 0.0000 | |
| 36 | W | A | 0.0000 | |
| 37 | N | A | 0.0000 | |
| 38 | A | A | 0.0000 | |
| 39 | N | A | -0.3173 | |
| 40 | T | A | 0.0000 | |
| 41 | Q | A | -1.4116 | |
| 42 | W | A | -1.8204 | |
| 43 | C | A | 0.0000 | |
| 44 | L | A | -1.7960 | |
| 45 | E | A | -2.7950 | |
| 46 | R | A | -3.1574 | |
| 47 | H | A | -2.8113 | |
| 48 | G | A | -2.0112 | |
| 49 | V | A | 0.0000 | |
| 50 | D | A | -2.2873 | |
| 51 | V | A | 0.0000 | |
| 52 | D | A | -1.4983 | |
| 53 | V | A | 0.0060 | |
| 54 | S | A | 0.0059 | |
| 55 | V | A | 0.2730 | |
| 56 | F | A | 0.0000 | |
| 57 | D | A | -0.1818 | |
| 58 | V | A | 0.0000 | |
| 59 | V | A | 1.1567 | |
| 60 | A | A | 0.2409 | |
| 61 | N | A | -0.1902 | |
| 62 | P | A | -0.0111 | |
| 63 | G | A | -0.5769 | |
| 64 | Q | A | -0.7898 | |
| 65 | T | A | -0.1348 | |
| 66 | F | A | 0.6712 | |
| 67 | R | A | -1.3007 | |
| 68 | G | A | -0.7940 | |
| 69 | P | A | -1.1160 | |
| 70 | D | A | -1.0179 | |
| 71 | M | A | -0.5432 | |
| 72 | T | A | -0.4095 | |
| 73 | I | A | 0.0000 | |
| 74 | F | A | 0.0000 | |
| 75 | Y | A | -0.1025 | |
| 76 | S | A | -0.4501 | |
| 77 | S | A | -0.3722 | |
| 78 | Q | A | -0.7434 | |
| 79 | L | A | 0.0000 | |
| 80 | G | A | -0.6670 | |
| 81 | T | A | 0.0101 | |
| 82 | Y | A | 0.0000 | |
| 83 | P | A | 0.0000 | |
| 84 | Y | A | 0.2162 | |
| 85 | Y | A | 0.0000 | |
| 86 | T | A | -0.6768 | |
| 87 | P | A | -0.6068 | |
| 88 | T | A | -0.7492 | |
| 89 | G | A | -1.0057 | |
| 90 | E | A | -1.6999 | |
| 91 | P | A | -0.5897 | |
| 92 | V | A | 0.7338 | |
| 93 | F | A | 1.3678 | |
| 94 | G | A | 0.1432 | |
| 95 | G | A | 0.0000 | |
| 96 | L | A | 0.0000 | |
| 97 | P | A | 0.0000 | |
| 98 | Q | A | -1.1627 | |
| 99 | N | A | -1.1025 | |
| 100 | A | A | 0.1225 | |
| 101 | S | A | 0.6376 | |
| 102 | L | A | 0.9676 | |
| 103 | I | A | 2.0145 | |
| 104 | A | A | 0.7206 | |
| 105 | H | A | 0.0000 | |
| 106 | L | A | 0.6438 | |
| 107 | A | A | -0.0861 | |
| 108 | R | A | -1.5893 | |
| 109 | T | A | 0.0000 | |
| 110 | F | A | 0.0502 | |
| 111 | Q | A | -1.0072 | |
| 112 | D | A | -0.7574 | |
| 113 | I | A | 0.0000 | |
| 114 | L | A | 0.4292 | |
| 115 | A | A | -0.0712 | |
| 116 | A | A | -0.4525 | |
| 117 | I | A | 0.0000 | |
| 118 | P | A | -0.6060 | |
| 119 | A | A | -0.7160 | |
| 120 | P | A | -1.3614 | |
| 121 | D | A | -2.5212 | |
| 122 | F | A | 0.0000 | |
| 123 | S | A | -1.6090 | |
| 124 | G | A | 0.0000 | |
| 125 | L | A | 0.0000 | |
| 126 | A | A | 0.0000 | |
| 127 | V | A | 0.0000 | |
| 128 | I | A | 0.0000 | |
| 129 | D | A | -0.7121 | |
| 130 | W | A | 0.0000 | |
| 131 | E | A | -1.9047 | |
| 132 | A | A | -0.9619 | |
| 133 | W | A | 0.0000 | |
| 134 | R | A | -0.8675 | |
| 135 | P | A | 0.0000 | |
| 136 | R | A | -0.8346 | |
| 137 | W | A | -0.5967 | |
| 138 | A | A | -0.1960 | |
| 139 | F | A | 0.0000 | |
| 140 | N | A | 0.0000 | |
| 141 | W | A | -0.7598 | |
| 142 | D | A | -1.7956 | |
| 143 | T | A | -1.0468 | |
| 144 | K | A | -1.2447 | |
| 145 | D | A | -1.2547 | |
| 146 | I | A | -0.9073 | |
| 147 | Y | A | 0.0000 | |
| 148 | R | A | -1.5848 | |
| 149 | Q | A | -1.8683 | |
| 150 | R | A | -1.4870 | |
| 151 | S | A | 0.0000 | |
| 152 | R | A | -2.2299 | |
| 153 | A | A | -1.3548 | |
| 154 | L | A | -0.9484 | |
| 155 | V | A | 0.0000 | |
| 156 | Q | A | -1.7708 | |
| 157 | A | A | -1.1551 | |
| 158 | Q | A | -1.6355 | |
| 159 | H | A | -1.6136 | |
| 160 | P | A | -1.6585 | |
| 161 | D | A | -2.1398 | |
| 162 | W | A | -1.2296 | |
| 163 | P | A | -1.0377 | |
| 164 | A | A | -1.0927 | |
| 165 | P | A | -1.1321 | |
| 166 | Q | A | -1.3279 | |
| 167 | V | A | 0.0000 | |
| 168 | E | A | -2.7124 | |
| 169 | A | A | -1.7002 | |
| 170 | V | A | -1.5391 | |
| 171 | A | A | 0.0000 | |
| 172 | Q | A | -2.3234 | |
| 173 | D | A | -2.6476 | |
| 174 | Q | A | -2.3773 | |
| 175 | F | A | 0.0000 | |
| 176 | Q | A | -1.7706 | |
| 177 | G | A | -1.8004 | |
| 178 | A | A | -1.4177 | |
| 179 | A | A | 0.0000 | |
| 180 | R | A | -1.7521 | |
| 181 | A | A | -0.7873 | |
| 182 | W | A | 0.0000 | |
| 183 | M | A | 0.0000 | |
| 184 | A | A | -0.7515 | |
| 185 | G | A | -0.5715 | |
| 186 | T | A | 0.0000 | |
| 187 | L | A | 0.0000 | |
| 188 | Q | A | -1.0807 | |
| 189 | L | A | -0.1249 | |
| 190 | G | A | 0.0000 | |
| 191 | R | A | -1.0423 | |
| 192 | A | A | -0.3876 | |
| 193 | L | A | -0.2598 | |
| 194 | R | A | 0.0000 | |
| 195 | P | A | -1.7396 | |
| 196 | R | A | -2.6518 | |
| 197 | G | A | 0.0000 | |
| 198 | L | A | 0.0000 | |
| 199 | W | A | 0.0000 | |
| 200 | G | A | 0.0000 | |
| 201 | F | A | 0.0000 | |
| 202 | Y | A | 0.0000 | |
| 203 | G | A | -0.4640 | |
| 204 | F | A | 0.0000 | |
| 205 | P | A | 0.0000 | |
| 206 | D | A | -0.3479 | |
| 207 | C | A | 0.0000 | |
| 208 | Y | A | 0.0000 | |
| 209 | N | A | 0.0000 | |
| 210 | Y | A | 0.0970 | |
| 211 | D | A | -1.3123 | |
| 212 | F | A | 0.0000 | |
| 213 | L | A | 1.0469 | |
| 214 | S | A | -0.2400 | |
| 215 | P | A | -0.7336 | |
| 216 | N | A | -1.3751 | |
| 217 | Y | A | -0.8570 | |
| 218 | T | A | -1.0003 | |
| 219 | G | A | 0.0000 | |
| 220 | Q | A | -1.4931 | |
| 221 | C | A | 0.0000 | |
| 222 | P | A | -0.8858 | |
| 223 | S | A | -0.7121 | |
| 224 | G | A | -0.8136 | |
| 225 | I | A | 0.0000 | |
| 226 | R | A | -1.7708 | |
| 227 | A | A | -1.3920 | |
| 228 | Q | A | -1.5080 | |
| 229 | N | A | 0.0000 | |
| 230 | D | A | -2.4302 | |
| 231 | Q | A | -2.0482 | |
| 232 | L | A | 0.0000 | |
| 233 | G | A | -1.0241 | |
| 234 | W | A | -0.6269 | |
| 235 | L | A | 0.0000 | |
| 236 | W | A | 0.0000 | |
| 237 | G | A | -0.6639 | |
| 238 | Q | A | -0.5776 | |
| 239 | S | A | 0.0000 | |
| 240 | R | A | -1.1300 | |
| 241 | A | A | 0.0000 | |
| 242 | L | A | 0.0000 | |
| 243 | Y | A | 0.0000 | |
| 244 | P | A | 0.0000 | |
| 245 | S | A | -0.0454 | |
| 246 | I | A | 0.0000 | |
| 247 | Y | A | 0.0419 | |
| 248 | M | A | 0.0000 | |
| 249 | P | A | 0.2980 | |
| 250 | A | A | 0.0863 | |
| 251 | V | A | 1.4702 | |
| 252 | L | A | 0.0000 | |
| 253 | E | A | -1.1339 | |
| 254 | G | A | -0.9512 | |
| 255 | T | A | -0.6812 | |
| 256 | G | A | -0.9628 | |
| 257 | K | A | -0.6400 | |
| 258 | S | A | 0.0000 | |
| 259 | Q | A | -0.9210 | |
| 260 | M | A | -0.5434 | |
| 261 | Y | A | 0.0000 | |
| 262 | V | A | 0.0000 | |
| 263 | Q | A | -0.7324 | |
| 264 | H | A | -0.6549 | |
| 265 | R | A | 0.0000 | |
| 266 | V | A | 0.0000 | |
| 267 | A | A | -0.3406 | |
| 268 | E | A | 0.0000 | |
| 269 | A | A | 0.0000 | |
| 270 | F | A | 0.2906 | |
| 271 | R | A | -0.1751 | |
| 272 | V | A | 0.0000 | |
| 273 | A | A | 0.0000 | |
| 274 | V | A | 1.3520 | |
| 275 | A | A | 0.3250 | |
| 276 | A | A | -0.2030 | |
| 277 | G | A | -0.3641 | |
| 278 | D | A | 0.0000 | |
| 279 | P | A | -0.1972 | |
| 280 | N | A | -0.8983 | |
| 281 | L | A | 0.0000 | |
| 282 | P | A | 0.0000 | |
| 283 | V | A | 0.0000 | |
| 284 | L | A | 0.0000 | |
| 285 | P | A | 0.0000 | |
| 286 | Y | A | 0.0000 | |
| 287 | V | A | 0.0000 | |
| 288 | Q | A | 0.0000 | |
| 289 | I | A | 0.0000 | |
| 290 | F | A | 0.0000 | |
| 291 | Y | A | 0.0000 | |
| 292 | D | A | -0.5515 | |
| 293 | T | A | -0.8313 | |
| 294 | T | A | -1.1383 | |
| 295 | N | A | -1.6986 | |
| 296 | H | A | -1.4606 | |
| 297 | F | A | -0.2884 | |
| 298 | L | A | 0.0000 | |
| 299 | P | A | -0.3256 | |
| 300 | L | A | 0.4697 | |
| 301 | D | A | -1.3370 | |
| 302 | E | A | 0.0000 | |
| 303 | L | A | 0.0000 | |
| 304 | E | A | -0.8283 | |
| 305 | H | A | -1.0654 | |
| 306 | S | A | 0.0000 | |
| 307 | L | A | 0.0000 | |
| 308 | G | A | 0.0000 | |
| 309 | E | A | 0.0000 | |
| 310 | S | A | 0.0000 | |
| 311 | A | A | 0.0000 | |
| 312 | A | A | 0.0000 | |
| 313 | Q | A | -0.3549 | |
| 314 | G | A | 0.0000 | |
| 315 | A | A | 0.0000 | |
| 316 | A | A | 0.0000 | |
| 317 | G | A | 0.0000 | |
| 318 | V | A | 0.0000 | |
| 319 | V | A | 0.0000 | |
| 320 | L | A | 0.0000 | |
| 321 | W | A | 0.4080 | |
| 322 | V | A | 0.0000 | |
| 323 | S | A | 0.0000 | |
| 324 | W | A | -0.4815 | |
| 325 | E | A | -2.0949 | |
| 326 | N | A | 0.0000 | |
| 327 | T | A | 0.0000 | |
| 328 | R | A | -2.9539 | |
| 329 | T | A | -2.7619 | |
| 330 | K | A | -3.2417 | |
| 331 | E | A | -3.1336 | |
| 332 | S | A | -2.5017 | |
| 333 | C | A | 0.0000 | |
| 334 | Q | A | -2.6607 | |
| 335 | A | A | -1.9522 | |
| 336 | I | A | 0.0000 | |
| 337 | K | A | -2.6545 | |
| 338 | E | A | -2.6806 | |
| 339 | Y | A | -1.3446 | |
| 340 | M | A | 0.0000 | |
| 341 | D | A | -1.8770 | |
| 342 | T | A | -0.8663 | |
| 343 | T | A | -0.2017 | |
| 344 | L | A | 0.0000 | |
| 345 | G | A | 0.0000 | |
| 346 | P | A | -0.1051 | |
| 347 | F | A | 0.0000 | |
| 348 | I | A | 0.0000 | |
| 349 | L | A | 0.3939 | |
| 350 | N | A | 0.0359 | |
| 351 | V | A | 0.0000 | |
| 352 | T | A | 0.0000 | |
| 353 | S | A | 0.0578 | |
| 354 | G | A | 0.0000 | |
| 355 | A | A | 0.0000 | |
| 356 | L | A | -0.4645 | |
| 357 | L | A | -0.2055 | |
| 358 | C | A | 0.0000 | |
| 359 | S | A | 0.0000 | |
| 360 | Q | A | -1.3554 | |
| 361 | A | A | -0.3049 | |
| 362 | L | A | -0.5778 | |
| 363 | C | A | 0.0000 | |
| 364 | S | A | -1.3094 | |
| 365 | G | A | -1.3266 | |
| 366 | H | A | -1.5857 | |
| 367 | G | A | 0.0000 | |
| 368 | R | A | -0.6427 | |
| 369 | C | A | 0.0000 | |
| 370 | V | A | -0.4343 | |
| 371 | R | A | 0.0000 | |
| 372 | R | A | -1.0353 | |
| 373 | T | A | -0.9511 | |
| 374 | S | A | -0.7464 | |
| 375 | H | A | -1.1452 | |
| 376 | P | A | -1.4884 | |
| 377 | K | A | -2.0573 | |
| 378 | A | A | -1.0028 | |
| 379 | L | A | -0.2978 | |
| 380 | L | A | 0.0000 | |
| 381 | L | A | 0.1625 | |
| 382 | L | A | 0.0000 | |
| 383 | N | A | -0.8734 | |
| 384 | P | A | -0.5393 | |
| 385 | A | A | -0.3523 | |
| 386 | S | A | -0.6902 | |
| 387 | F | A | 0.0000 | |
| 388 | S | A | -0.9285 | |
| 389 | I | A | -0.3262 | |
| 390 | Q | A | -0.8146 | |
| 391 | L | A | -0.0741 | |
| 392 | T | A | -0.3100 | |
| 393 | P | A | -0.6504 | |
| 394 | G | A | -0.7899 | |
| 395 | G | A | -0.8216 | |
| 396 | G | A | -0.6368 | |
| 397 | P | A | -0.4257 | |
| 398 | L | A | -0.2648 | |
| 399 | S | A | -0.2498 | |
| 400 | L | A | -0.4858 | |
| 401 | R | A | -1.9055 | |
| 402 | G | A | -1.1474 | |
| 403 | A | A | -0.2283 | |
| 404 | L | A | 0.1165 | |
| 405 | S | A | -0.1849 | |
| 406 | L | A | 0.3346 | |
| 407 | E | A | -1.6712 | |
| 408 | D | A | -1.2238 | |
| 409 | Q | A | -0.7193 | |
| 410 | A | A | -0.8336 | |
| 411 | Q | A | -1.3392 | |
| 412 | M | A | 0.0000 | |
| 413 | A | A | -0.3876 | |
| 414 | V | A | 0.3149 | |
| 415 | E | A | -0.6612 | |
| 416 | F | A | 0.0000 | |
| 417 | K | A | -0.9624 | |
| 418 | C | A | -0.6526 | |
| 419 | R | A | -0.7711 | |
| 420 | C | A | -0.8620 | |
| 421 | Y | A | 0.0000 | |
| 422 | P | A | -0.8898 | |
| 423 | G | A | -0.6426 | |
| 424 | W | A | -1.4874 | |
| 425 | Q | A | -1.6827 | |
| 426 | A | A | -1.1010 | |
| 427 | P | A | -0.7439 | |
| 428 | W | A | -0.7655 | |
| 429 | C | A | 0.0000 | |
| 430 | E | A | -3.0823 | |
| 431 | R | A | -3.4912 | |
| 432 | K | A | -2.7136 | |
| 433 | S | A | -0.8722 | |
| 434 | M | A | 0.9156 | |
| 435 | W | A | 1.4165 | |
| 436 | T | A | 0.4257 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine