Project name: SH3_W119H

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA119H
Energy difference between WT (input) and mutated protein (by FoldX)	2.44208 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1025
• Maximal score value: 1.7964
• Average score: -1.0391
• Total score value: -59.2268

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9237
88	V	A	0.4309
89	A	A	0.0000
90	L	A	-0.1489
91	Y	A	-0.5705
92	D	A	-2.5520
93	Y	A	-1.9150
94	E	A	-2.6374
95	A	A	-2.6214
96	R	A	-2.9833
97	T	A	-2.6608
98	E	A	-3.1025
99	D	A	-3.0425
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2007
103	F	A	0.0000
104	H	A	-2.7249
105	K	A	-2.4036
106	G	A	-1.4592
107	E	A	-1.3102
108	K	A	-0.6438
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0565
112	L	A	0.1433
113	N	A	-1.0432
114	S	A	-1.4107
115	S	A	-1.7377
116	E	A	-2.7715
117	G	A	-2.4547
118	D	A	-2.9784
119	H	A	-2.1933	mutated: WA119H
120	W	A	-1.5997
121	E	A	-1.4080
122	A	A	0.0000
123	R	A	-1.7150
124	S	A	0.0000
125	L	A	0.0587
126	T	A	-0.4736
127	T	A	-0.8209
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.6920
131	G	A	-1.6274
132	Y	A	-1.1932
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-1.2734
136	N	A	-1.3137
137	Y	A	-0.2361
138	V	A	0.0000
139	A	A	0.4162
140	P	A	0.7757
141	V	A	1.7964