Project name: SH3_G128Q

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA128Q
Energy difference between WT (input) and mutated protein (by FoldX)	3.31461 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7964
• Average score: -0.9913
• Total score value: -56.5058

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5187
86	L	A	0.8225
87	F	A	0.9261
88	V	A	0.4328
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3046
108	K	A	-0.5996
109	F	A	0.0000
110	Q	A	-0.5181
111	I	A	0.0045
112	L	A	0.1874
113	N	A	-0.8910
114	S	A	-1.1812
115	S	A	-1.5972
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0295
121	E	A	-1.1026
122	A	A	0.0000
123	R	A	-1.7322
124	S	A	0.0000
125	L	A	-0.1356
126	T	A	-0.6655
127	T	A	-1.1974
128	Q	A	-2.1596	mutated: GA128Q
129	E	A	-2.5867
130	T	A	-1.8039
131	G	A	-1.5333
132	Y	A	-0.8333
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7764
141	V	A	1.7964