Project name: Genomica [mutate: WH96C]

Chain sequence(s)	A: GREFGNLTRMRHVISYSLSPFEQRAYPHVFTKGIPNVLRRIRESFFRVVPQFVVFYLIYTWGTEEFERSKRKNPAAYENDK; C: SHTDIKVPDFSEYRRLEVLDSTKSSRESSEARKGFSYLVTGVTTVGVAYAAKNAVTQFVSSMSASADVLALAKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTQKEIEQEAAVELSQLRDPQHDLDRVKKPEWVILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRLGPAPLNLEVPTYEFTSDDMVIVG; B: MLSVASRSGPFAPVLSATSRGVAGALRPLVQATVPATPEQPVLDLKRPFLSRESLSG; E: EEEEEEEELVDPLTTVREQCEQLEKCVKARERLELCDERVSSRSHTEEDCTEELFDFLHARDHCVAHKLFNNLK; D: AAATLTSKLYSLLFRRTSTFALTIIVGVMFFERAFDQGADAIYDHINEGKLWKHIKHKYENK; G: VTRFLGPRYRELVKNWVPTAYTWGAVGAVGLVWATDWRLILDWVPYINGKF; F: AVSASGKWLDGIRKWYYNAAGFNKLGLMRDDTIYEDEDVKEAIRRLPENLYNDRMFRIKRALDLNLKHQILPKEQWTKYEEENFYLEPYLKEVIRERKEREEWAKK; H: SDLELHPPSYPWSHRGLLSSLDHTSIRRGFQVYKQVCASCHSMDFVAYRHLVGVCYTEDEAKELAAEVEVQDGPNEDGEMFMRPGKLFDYFPKPYPNSEAARAANNGALPPDLSYIVRARHGGEDYVFSLLTGYCEPPTGVSLREGLYFNPYFPGQAIAMAPPIYTDVLEFDDGTPATMSQIAKDVCTFLRWASEPEHDHRKRMGLKMLMMMALLVPLVYTIKRHKWSVLKSRKLAYRPPK; K: PQDLEFTKLPNGLVIASLENYSPVSRIGLFIKAGSRYEDFSNLGTTHLLRLTSSLTTKGASSFKITRGIEAVGGKLSVTATRENMAYTVECLRGDVDILMEFLLNVTTAPEFRRWEVADLQPQLKIDKAVAFQNPQTHVIENLHAAAYRNALANPLYCPDYRIGKVTSEELHYFVQNHFTSARMALIGLGVSHPVLKQVAEQFLNMRGGLGLSGAKANYRGGEIREQNGDSLVHAAFVAESAVAGSAEANAFSVLQHVLGAGPHVKRGSNTTSHLHQAVAKATQQPFDVSAFNASYSDSGLFGIYTISQATAAGDVIKAAYNQVKTIAQGNLSNTDVQAAKNKLKAGYLMSVESSECFLEEVGSQALVAGSYMPPSTVLQQIDSVANADIINAAKKFVSGQKSMAASGNLGHTPFVDEL; J: TPMRKTNPLMKLINHSFIDLPTPSNISAWWNFGSLLGACLILQITTGLFLAMHYSPDASTAFSSIAHITRDVNYGWIIRYLHANGASMFFICLFLHIGRGLYYGSFLYSETWNIGIILLLATMATAFMGYVLPWGQMSFWGATVITNLLSAIPYIGTDLVQWIWGGYSVDSPTLTRFFTFHFILPFIIAALATLHLLFLHETGSNNPLGITSHSDKITFHPYYTIKDALGLLLFLLSLMTLTLFSPDLLGDPDNYTLANPLNTPPHIKPEWYFLFAYTILRSVPNKLGGVLALLLSILILAMIPILHMSKQQSMMFRPLSQSLYWLLAADLLILTWIGGQPVSYPFTIIGQVASVLYFTTILILMPTISLIENKMLKW; L: TATFAQALQFVPETQVSLLDNGLRVASEQSSQPTCTVGVWIDVGSRFETEKNNGAGYFLEHLAFKGTKNRPGSALEKEVESMGAHLNAYSTREHTAYYIKALSKDLPKAVELLGDIVQNCSLEDSQIEKERDVILREMQENDASMRDVVFNYLHATAFQGTPLAQAVEGPSENVRKLSRADLTEYLSTHYKAPRMVLAAAGGVEHQQLLDLAQKHLGGIPWTYAEDAVPTLTPCRFTGSEIRHRDDALPFAHVAIAVEGPGWASPDNVALQVANAIIGHYDCTYGGGVHLSSPLASGAVANKLCQSFQTFSICYAETGLLGAHFVCDRMKIDDMMFVLQGQWMRLCTSATESEVARGKNILRNALVSHLDGTTPVCEDIGRSLLTYGRRIPLAEWESRIAEVDASVVREICSKYIYDQCPAVAGYGPIEQLPDYNRIRSGMFWLRF; O: MLSVASRSGPFAPVLSATSRGVAGALRPLVQATVPATPEQPVLDLKRPFLSRESLSG; N: GREFGNLTRMRHVISYSLSPFEQRAYPHVFTKGIPNVLRRIRESFFRVVPQFVVFYLIYTWGTEEFERSKRKNPAAYENDK; Q: AAATLTSKLYSLLFRRTSTFALTIIVGVMFFERAFDQGADAIYDHINEGKLWKHIKHKYENK; P: SHTDIKVPDFSEYRRLEVLDSTKSSRESSEARKGFSYLVTGVTTVGVAYAAKNAVTQFVSSMSASADVLALAKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTQKEIEQEAAVELSQLRDPQHDLDRVKKPEWVILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRLGPAPLNLEVPTYEFTSDDMVIVG; S: AVSASGKWLDGIRKWYYNAAGFNKLGLMRDDTIYEDEDVKEAIRRLPENLYNDRMFRIKRALDLNLKHQILPKEQWTKYEEENFYLEPYLKEVIRERKEREEWAKK; R: EEEEEEEELVDPLTTVREQCEQLEKCVKARERLELCDERVSSRSHTEEDCTEELFDFLHARDHCVAHKLFNNLK; U: SDLELHPPSYPWSHRGLLSSLDHTSIRRGFQVYKQVCASCHSMDFVAYRHLVGVCYTEDEAKELAAEVEVQDGPNEDGEMFMRPGKLFDYFPKPYPNSEAARAANNGALPPDLSYIVRARHGGEDYVFSLLTGYCEPPTGVSLREGLYFNPYFPGQAIAMAPPIYTDVLEFDDGTPATMSQIAKDVCTFLRWASEPEHDHRKRMGLKMLMMMALLVPLVYTIKRHKWSVLKSRKLAYRPPK; T: VTRFLGPRYRELVKNWVPTAYTWGAVGAVGLVWATDWRLILDWVPYINGKF; W: PQDLEFTKLPNGLVIASLENYSPVSRIGLFIKAGSRYEDFSNLGTTHLLRLTSSLTTKGASSFKITRGIEAVGGKLSVTATRENMAYTVECLRGDVDILMEFLLNVTTAPEFRRWEVADLQPQLKIDKAVAFQNPQTHVIENLHAAAYRNALANPLYCPDYRIGKVTSEELHYFVQNHFTSARMALIGLGVSHPVLKQVAEQFLNMRGGLGLSGAKANYRGGEIREQNGDSLVHAAFVAESAVAGSAEANAFSVLQHVLGAGPHVKRGSNTTSHLHQAVAKATQQPFDVSAFNASYSDSGLFGIYTISQATAAGDVIKAAYNQVKTIAQGNLSNTDVQAAKNKLKAGYLMSVESSECFLEEVGSQALVAGSYMPPSTVLQQIDSVANADIINAAKKFVSGQKSMAASGNLGHTPFVDEL; V: TPMRKTNPLMKLINHSFIDLPTPSNISAWWNFGSLLGACLILQITTGLFLAMHYSPDASTAFSSIAHITRDVNYGWIIRYLHANGASMFFICLFLHIGRGLYYGSFLYSETWNIGIILLLATMATAFMGYVLPWGQMSFWGATVITNLLSAIPYIGTDLVQWIWGGYSVDSPTLTRFFTFHFILPFIIAALATLHLLFLHETGSNNPLGITSHSDKITFHPYYTIKDALGLLLFLLSLMTLTLFSPDLLGDPDNYTLANPLNTPPHIKPEWYFLFAYTILRSVPNKLGGVLALLLSILILAMIPILHMSKQQSMMFRPLSQSLYWLLAADLLILTWIGGQPVSYPFTIIGQVASVLYFTTILILMPTISLIENKMLKW; Y: TATFAQALQFVPETQVSLLDNGLRVASEQSSQPTCTVGVWIDVGSRFETEKNNGAGYFLEHLAFKGTKNRPGSALEKEVESMGAHLNAYSTREHTAYYIKALSKDLPKAVELLGDIVQNCSLEDSQIEKERDVILREMQENDASMRDVVFNYLHATAFQGTPLAQAVEGPSENVRKLSRADLTEYLSTHYKAPRMVLAAAGGVEHQQLLDLAQKHLGGIPWTYAEDAVPTLTPCRFTGSEIRHRDDALPFAHVAIAVEGPGWASPDNVALQVANAIIGHYDCTYGGGVHLSSPLASGAVANKLCQSFQTFSICYAETGLLGAHFVCDRMKIDDMMFVLQGQWMRLCTSATESEVARGKNILRNALVSHLDGTTPVCEDIGRSLLTYGRRIPLAEWESRIAEVDASVVREICSKYIYDQCPAVAGYGPIEQLPDYNRIRSGMFWLRF
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WH96C
Energy difference between WT (input) and mutated protein (by FoldX)	4.09399 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -6.2011
• Maximal score value: 3.6043
• Average score: -0.443
• Total score value: -1870.4608

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
2	G	A	-2.0751
3	R	A	-3.1066
4	E	A	-2.1896
5	F	A	-1.3035
6	G	A	0.0000
7	N	A	-2.2840
8	L	A	-1.6201
9	T	A	-1.3603
10	R	A	-2.2111
11	M	A	0.0000
12	R	A	-1.5350
13	H	A	0.0000
14	V	A	0.0000
15	I	A	0.0000
16	S	A	-0.1817
17	Y	A	0.0000
18	S	A	0.0000
19	L	A	0.0000
20	S	A	0.0000
21	P	A	0.0000
22	F	A	0.0000
23	E	A	0.0000
24	Q	A	0.0000
25	R	A	-0.4858
26	A	A	-0.0562
27	Y	A	0.8204
28	P	A	-0.0612
29	H	A	0.0875
30	V	A	1.4091
31	F	A	2.0231
32	T	A	0.4133
33	K	A	-0.3681
34	G	A	0.9776
35	I	A	1.7754
36	P	A	0.8665
37	N	A	0.2834
38	V	A	1.2609
39	L	A	0.7674
40	R	A	-0.6452
41	R	A	-1.0818
42	I	A	0.3945
43	R	A	-1.3908
44	E	A	-1.4691
45	S	A	-0.1373
46	F	A	1.1622
47	F	A	1.8751
48	R	A	0.4990
49	V	A	1.3716
50	V	A	1.8677
51	P	A	1.6816
52	Q	A	1.7414
53	F	A	2.1964
54	V	A	2.8308
55	V	A	3.2775
56	F	A	3.0680
57	Y	A	3.5576
58	L	A	3.6043
59	I	A	2.6913
60	Y	A	2.4881
61	T	A	1.3364
62	W	A	0.9274
63	G	A	0.0000
64	T	A	-0.9346
65	E	A	-2.2612
66	E	A	-2.1466
67	F	A	-2.0691
68	E	A	-3.9078
69	R	A	-4.1539
70	S	A	-2.6028
71	K	A	-3.0701
72	R	A	-3.8117
73	K	A	-3.8280
74	N	A	-2.5368
75	P	A	-2.0373
76	A	A	-2.4376
77	A	A	-2.2521
78	Y	A	-3.0181
79	E	A	-3.9145
80	N	A	-3.5271
81	D	A	-4.1238
82	K	A	-3.7562
1	M	B	0.3473
2	L	B	0.3679
3	S	B	0.0000
4	V	B	0.1971
5	A	B	0.1114
6	S	B	0.0806
7	R	B	-0.1152
8	S	B	-0.0819
9	G	B	0.0000
10	P	B	0.0000
11	F	B	0.5417
12	A	B	0.3955
13	P	B	0.4166
14	V	B	0.5237
15	L	B	0.3512
16	S	B	-0.0755
17	A	B	-0.1569
18	T	B	-0.3611
19	S	B	-0.5439
20	R	B	0.1258
21	G	B	0.6135
22	V	B	1.8616
23	A	B	0.7921
24	G	B	0.2072
25	A	B	0.2738
26	L	B	0.3700
27	R	B	0.0000
28	P	B	0.0000
29	L	B	1.3392
30	V	B	1.0807
31	Q	B	0.0000
32	A	B	0.5891
33	T	B	0.0313
34	V	B	0.3239
35	P	B	0.0000
36	A	B	-1.0210
37	T	B	-1.4539
38	P	B	-1.4693
39	E	B	-2.8119
40	Q	B	-2.3890
41	P	B	-1.5746
42	V	B	-0.6479
43	L	B	0.0000
44	D	B	-1.5538
45	L	B	-0.6087
46	K	B	-0.5338
47	R	B	-0.1894
48	P	B	0.3946
49	F	B	1.6452
50	L	B	1.6839
51	S	B	0.1115
52	R	B	-1.5741
53	E	B	-1.9449
54	S	B	-1.2214
55	L	B	-0.6091
56	S	B	-0.4381
57	G	B	-0.0628
79	S	C	-1.7905
80	H	C	0.0000
81	T	C	0.0000
82	D	C	-2.9418
83	I	C	0.0000
84	K	C	-2.6731
85	V	C	0.0000
86	P	C	-1.5551
87	D	C	-2.5502
88	F	C	0.0000
89	S	C	-1.4571
90	E	C	-2.0438
91	Y	C	-0.8653
92	R	C	0.0000
93	R	C	-0.2055
94	L	C	0.8214
95	E	C	-0.4526
96	V	C	0.0000
97	L	C	-0.1267
98	D	C	-1.5833
99	S	C	-1.3701
100	T	C	-1.3492
101	K	C	-2.3947
102	S	C	-1.8784
103	S	C	0.0000
104	R	C	-2.1869
105	E	C	-2.7103
106	S	C	0.0000
107	S	C	0.0000
108	E	C	-1.4513
109	A	C	0.0000
110	R	C	0.0000
111	K	C	0.0000
112	G	C	0.0000
113	F	C	0.8993
114	S	C	0.8093
115	Y	C	0.9070
116	L	C	1.3019
117	V	C	1.2327
118	T	C	0.8695
119	G	C	0.0000
120	V	C	1.4025
121	T	C	0.0000
122	T	C	0.8998
123	V	C	1.0741
124	G	C	0.0000
125	V	C	1.3601
126	A	C	0.7194
127	Y	C	0.0000
128	A	C	0.5265
129	A	C	0.6246
130	K	C	0.4083
131	N	C	0.0304
132	A	C	0.6991
133	V	C	1.8134
134	T	C	0.9779
135	Q	C	0.6908
136	F	C	1.1960
137	V	C	1.4547
138	S	C	0.6709
139	S	C	0.0000
140	M	C	0.0000
141	S	C	-0.0066
142	A	C	0.3598
143	S	C	0.0000
144	A	C	0.4363
145	D	C	0.6910
146	V	C	1.1941
147	L	C	1.7120
148	A	C	1.4461
149	L	C	1.6491
150	A	C	0.0000
151	K	C	-0.1027
152	I	C	0.9923
153	E	C	0.4633
154	I	C	1.3311
155	K	C	0.0000
156	L	C	0.3067
157	S	C	-1.0491
158	D	C	-1.9195
159	I	C	0.0000
160	P	C	-1.9779
161	E	C	-2.8268
162	G	C	-1.9066
163	K	C	-1.9566
164	N	C	0.0000
165	M	C	0.2494
166	A	C	0.0522
167	F	C	-0.4778
168	K	C	-1.9888
169	W	C	0.0000
170	R	C	-1.3551
171	G	C	-1.3405
172	K	C	-1.3550
173	P	C	-0.7263
174	L	C	0.0000
175	F	C	0.2665
176	V	C	0.0000
177	R	C	0.0000
178	H	C	-1.6602
179	R	C	0.0000
180	T	C	-2.2489
181	Q	C	-3.0023
182	K	C	-3.3843
183	E	C	-2.5042
184	I	C	-2.0804
185	E	C	-3.2889
186	Q	C	-2.5838
187	E	C	0.0000
188	A	C	-0.9929
189	A	C	-1.0052
190	V	C	-1.0832
191	E	C	-1.3924
192	L	C	-0.4551
193	S	C	-1.1940
194	Q	C	-2.0098
195	L	C	0.0000
196	R	C	-2.0037
197	D	C	-1.2655
198	P	C	-1.2004
199	Q	C	-1.1474
200	H	C	-0.9960
201	D	C	0.0000
202	L	C	-0.4824
203	D	C	-2.0813
204	R	C	-1.5942
205	V	C	0.0000
206	K	C	-2.8721
207	K	C	-2.6523
208	P	C	-1.7111
209	E	C	-1.7977
210	W	C	-1.0716
211	V	C	0.0000
212	I	C	0.0000
213	L	C	0.0000
214	I	C	0.0000
215	G	C	0.0000
216	V	C	0.1903
217	C	C	0.0000
218	T	C	0.2480
219	H	C	0.3248
220	L	C	0.7212
221	G	C	0.5044
222	C	C	0.0000
223	V	C	1.1848
224	P	C	0.0000
225	I	C	1.4125
226	A	C	0.3986
227	N	C	-0.6060
228	A	C	-0.3225
229	G	C	-0.7650
230	D	C	-1.6332
231	F	C	-0.6122
232	G	C	-0.9038
233	G	C	0.0000
234	Y	C	0.0000
235	Y	C	0.3063
236	C	C	0.0000
237	P	C	-0.3138
238	C	C	0.0000
239	H	C	-0.6616
240	G	C	-0.4925
241	S	C	0.0000
242	H	C	-0.1474
243	Y	C	0.0000
244	D	C	0.0000
245	A	C	0.0000
246	S	C	0.0000
247	G	C	0.0000
248	R	C	0.0000
249	I	C	0.0000
250	R	C	-0.4884
251	L	C	0.4699
252	G	C	0.0748
253	P	C	0.0364
254	A	C	0.0000
255	P	C	-0.0237
256	L	C	0.4819
257	N	C	0.0000
258	L	C	0.0000
259	E	C	-1.4389
260	V	C	-0.6528
261	P	C	0.0000
262	T	C	-0.7677
263	Y	C	-0.9744
264	E	C	-1.8306
265	F	C	-1.0285
266	T	C	-0.7710
267	S	C	-1.4289
268	D	C	-2.3777
269	D	C	-2.3081
270	M	C	-0.6919
271	V	C	0.0000
272	I	C	0.2662
273	V	C	0.0000
274	G	C	-0.9326
2	A	D	-0.1190
3	A	D	0.2892
4	A	D	-0.0718
5	T	D	0.1567
6	L	D	0.9973
7	T	D	0.0000
8	S	D	-0.5861
9	K	D	-0.8449
10	L	D	0.4254
11	Y	D	0.0000
12	S	D	0.0254
13	L	D	1.2236
14	L	D	1.0176
15	F	D	0.0000
16	R	D	-0.3003
17	R	D	0.0461
18	T	D	0.2330
19	S	D	0.0000
20	T	D	0.6269
21	F	D	0.6782
22	A	D	0.7778
23	L	D	0.8089
24	T	D	0.7240
25	I	D	0.9646
26	I	D	1.0901
27	V	D	1.2954
28	G	D	1.1185
29	V	D	0.0000
30	M	D	0.0000
31	F	D	2.1675
32	F	D	1.1173
33	E	D	0.4226
34	R	D	0.0000
35	A	D	0.3097
36	F	D	0.0101
37	D	D	-0.5771
38	Q	D	-1.1759
39	G	D	-0.5979
40	A	D	0.0000
41	D	D	-0.8919
42	A	D	-0.2326
43	I	D	0.4171
44	Y	D	0.0000
45	D	D	-1.0098
46	H	D	-0.7590
47	I	D	0.4624
48	N	D	0.0000
49	E	D	-2.2582
50	G	D	-1.8264
51	K	D	-1.4171
52	L	D	0.0000
53	W	D	-1.4389
54	K	D	-2.2034
55	H	D	-2.3205
56	I	D	-1.9235
57	K	D	-2.3713
58	H	D	-2.8204
59	K	D	-2.4608
60	Y	D	-1.5028
61	E	D	-3.0473
62	N	D	-3.0300
63	K	D	-2.8425
18	E	E	-3.8217
19	E	E	-5.4991
20	E	E	-5.9878
21	E	E	-6.2011
22	E	E	-5.9855
23	E	E	-5.8659
24	E	E	-5.2432
25	E	E	-4.4283
26	L	E	-2.0503
27	V	E	-0.0328
28	D	E	0.0000
29	P	E	0.1832
30	L	E	0.0000
31	T	E	-0.4367
32	T	E	-0.7159
33	V	E	0.0000
34	R	E	-1.9300
35	E	E	-2.8149
36	Q	E	-2.5049
37	C	E	0.0000
38	E	E	-2.4337
39	Q	E	-2.3794
40	L	E	-1.6305
41	E	E	-2.5632
42	K	E	-2.4744
43	C	E	0.0000
44	V	E	-2.3790
45	K	E	-3.4846
46	A	E	0.0000
47	R	E	-3.1868
48	E	E	-3.7425
49	R	E	-3.8883
50	L	E	-3.0338
51	E	E	-3.6737
52	L	E	-2.3614
53	C	E	0.0000
54	D	E	-2.7645
55	E	E	-3.1676
56	R	E	-2.2843
57	V	E	0.0000
58	S	E	-2.0274
59	S	E	-2.1235
60	R	E	-2.9100
61	S	E	-1.8731
62	H	E	-2.1898
63	T	E	-2.6805
64	E	E	-3.0606
65	E	E	0.0000
66	D	E	-1.0977
67	C	E	0.0000
68	T	E	0.0000
69	E	E	-1.2787
70	E	E	-1.8047
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