Project name: SH3_T130R

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	TA130R
Energy difference between WT (input) and mutated protein (by FoldX)	-0.905905 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.4539
• Maximal score value: 1.7964
• Average score: -1.0768
• Total score value: -61.3765

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4777
86	L	A	0.7271
87	F	A	0.8784
88	V	A	0.4084
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6269
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1034
99	D	A	-3.2715
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.3941
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3042
108	K	A	-0.6761
109	F	A	0.0000
110	Q	A	-0.8698
111	I	A	-0.3139
112	L	A	-0.0705
113	N	A	-0.8266
114	S	A	-1.1346
115	S	A	-1.5707
116	E	A	-2.5368
117	G	A	-2.1183
118	D	A	-2.4305
119	W	A	-1.0770
120	W	A	-1.0120
121	E	A	-1.4614
122	A	A	0.0000
123	R	A	-2.4955
124	S	A	0.0000
125	L	A	-0.1173
126	T	A	-0.4248
127	T	A	-0.9898
128	G	A	-1.6871
129	E	A	-3.0526
130	R	A	-3.4539	mutated: TA130R
131	G	A	-2.3292
132	Y	A	-1.2023
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9102
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7561
141	V	A	1.7964