Project name: 3SRF pyruvate kinsea chain A

Chain sequence(s)	A: IQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAMFHRKLFEELVRASSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP
Distance of aggregation	10 Å
Dynamic mode	No

• Minimal score value: -3.8901
• Maximal score value: 1.844
• Average score: -0.8297
• Total score value: -428.9686

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
12	I	A	1.0207
13	Q	A	-0.4346
14	T	A	-0.4993
15	Q	A	-0.9999
16	Q	A	-0.6200
17	L	A	0.0000
18	H	A	-0.7433
19	A	A	0.0000
20	A	A	0.0000
21	M	A	-0.4396
22	A	A	-0.7593
23	D	A	-1.5862
24	T	A	0.0738
25	F	A	1.7508
26	L	A	1.5283
27	E	A	-0.5109
28	H	A	0.0000
29	M	A	0.8973
30	C	A	0.3203
31	R	A	-0.8728
32	L	A	-0.1806
33	D	A	-0.1117
34	I	A	0.5701
35	D	A	-0.7262
36	S	A	0.0000
37	P	A	-0.6250
38	P	A	-0.6234
39	I	A	-0.0346
40	T	A	0.0048
41	A	A	0.0000
42	R	A	0.0000
43	N	A	-0.2141
44	T	A	0.0000
45	G	A	0.0000
46	I	A	0.0000
47	I	A	0.0000
48	C	A	0.0000
49	T	A	0.0000
50	I	A	0.0000
51	G	A	0.0000
52	P	A	-0.8266
53	A	A	-1.1185
54	S	A	0.0000
55	R	A	-0.9968
56	S	A	-0.9655
57	V	A	-1.4313
58	E	A	-2.3823
59	T	A	-1.8302
60	L	A	0.0000
61	K	A	-2.6418
62	E	A	-3.0452
63	M	A	0.0000
64	I	A	0.0000
65	K	A	-2.6366
66	S	A	0.0000
67	G	A	0.0000
68	M	A	0.0000
69	N	A	0.0000
70	V	A	0.0000
71	A	A	0.0000
72	R	A	0.0000
73	L	A	0.0000
74	N	A	-0.6165
75	F	A	0.0000
76	S	A	-0.9126
77	H	A	-1.1653
78	G	A	-1.2011
79	T	A	-1.1284
80	H	A	-1.6803
81	E	A	-2.3306
82	Y	A	-1.4211
83	H	A	0.0000
84	A	A	-1.8807
85	E	A	-2.7654
86	T	A	0.0000
87	I	A	0.0000
88	K	A	-2.5576
89	N	A	-1.9151
90	V	A	0.0000
91	R	A	-1.8749
92	T	A	-1.6078
93	A	A	0.0000
94	T	A	0.0000
95	E	A	-2.1796
96	S	A	-1.1537
97	F	A	-0.7787
98	A	A	-0.8845
99	S	A	-0.8506
100	D	A	-0.9796
101	P	A	-0.5831
102	I	A	0.1605
103	L	A	0.6571
104	Y	A	0.0000
105	R	A	-0.4939
106	P	A	0.0000
107	V	A	0.0000
108	A	A	0.0000
109	V	A	0.0000
110	A	A	0.0000
111	L	A	0.0000
112	D	A	0.0000
113	T	A	0.0000
114	K	A	-0.7906
115	G	A	-0.5803
116	P	A	-0.6455
117	E	A	-0.8042
118	I	A	0.0000
119	R	A	-1.2788
120	T	A	0.0000
121	G	A	0.0000
122	L	A	-1.3244
123	I	A	-1.9800
124	K	A	-2.8133
125	G	A	-1.8299
128	T	A	-0.6653
129	A	A	-1.3068
130	E	A	-2.5841
131	V	A	-2.4322
132	E	A	-3.2971
133	L	A	0.0000
134	K	A	-3.6767
135	K	A	-3.6137
136	G	A	-2.4367
137	A	A	-2.2096
138	T	A	-1.8625
139	L	A	0.0000
140	K	A	-1.4539
141	I	A	0.0000
142	T	A	0.0000
143	L	A	0.0000
144	D	A	-2.2020
145	N	A	-1.9693
146	A	A	-1.0682
147	Y	A	-1.1471
148	M	A	-1.2107
149	E	A	-2.5406
150	K	A	-2.4411
151	C	A	0.0000
152	D	A	-2.6121
153	E	A	-3.2547
154	N	A	-2.2004
155	I	A	-1.0612
156	L	A	0.0000
157	W	A	0.0000
158	L	A	0.0000
159	D	A	-1.8330
160	Y	A	0.0000
161	K	A	-2.8924
162	N	A	-2.1807
163	I	A	0.0000
164	C	A	-1.6576
165	K	A	-2.4220
166	V	A	-1.3622
167	V	A	0.0000
168	E	A	-1.9562
169	V	A	-0.2605
170	G	A	-1.1081
171	S	A	-1.1106
172	K	A	-1.5547
173	I	A	0.0000
174	Y	A	-0.4020
175	V	A	0.0000
176	D	A	-1.1805
177	D	A	-1.6847
178	G	A	-0.4514
179	L	A	0.5636
180	I	A	0.0000
181	S	A	-0.8796
182	L	A	0.0000
183	Q	A	-1.7866
184	V	A	0.0000
185	K	A	-2.3942
186	Q	A	-2.2451
187	K	A	-1.9646
188	G	A	-0.9762
189	A	A	-0.9737
190	D	A	-2.1482
191	F	A	-0.7376
192	L	A	0.0000
193	V	A	-1.3499
194	T	A	0.0000
195	E	A	-2.4660
196	V	A	0.0000
197	E	A	-2.6435
198	N	A	-2.0335
199	G	A	-2.2248
200	G	A	-1.2962
201	S	A	-1.7355
202	L	A	0.0000
203	G	A	-1.2274
204	S	A	-1.2412
205	K	A	-1.2414
206	K	A	-1.4418
207	G	A	-1.3985
208	V	A	0.0000
209	N	A	0.0000
210	L	A	0.0000
211	P	A	-0.6333
212	G	A	-1.0144
213	A	A	-0.9138
214	A	A	-0.6076
215	V	A	-0.7755
216	D	A	-1.7099
217	L	A	-0.7656
218	P	A	-0.6224
219	A	A	-0.7204
220	V	A	-0.5618
221	S	A	-1.0502
222	E	A	-2.1275
223	K	A	-1.6892
224	D	A	0.0000
225	I	A	-1.0840
226	Q	A	-1.8663
227	D	A	0.0000
228	L	A	0.0000
229	K	A	-2.3766
230	F	A	-1.6292
231	G	A	0.0000
232	V	A	-2.5984
233	E	A	-2.9692
234	Q	A	-2.2742
235	D	A	-2.8518
236	V	A	0.0000
237	D	A	0.0000
238	M	A	0.0000
239	V	A	0.0000
240	F	A	0.0000
241	A	A	0.0000
242	S	A	0.0000
243	F	A	-0.2742
244	I	A	0.0000
245	R	A	-1.4465
246	K	A	-1.9526
247	A	A	-1.7209
248	S	A	-1.2089
249	D	A	0.0000
250	V	A	0.0000
251	H	A	-2.3343
252	E	A	-2.3528
253	V	A	0.0000
254	R	A	-2.7227
255	K	A	-3.1412
256	V	A	0.0000
257	L	A	0.0000
258	G	A	-3.1596
259	E	A	-3.7930
260	K	A	-3.8901
261	G	A	0.0000
262	K	A	-3.7712
263	N	A	-2.8488
264	I	A	0.0000
265	K	A	-0.9844
266	I	A	0.0000
267	I	A	0.0000
268	S	A	0.0000
269	K	A	0.0000
270	I	A	0.0000
271	E	A	0.0000
272	N	A	-1.1429
273	H	A	-1.7595
274	E	A	-2.3302
275	G	A	0.0000
276	V	A	-1.7567
277	R	A	-3.0610
278	R	A	-2.8258
279	F	A	0.0000
280	D	A	-3.2935
281	E	A	-2.6450
282	I	A	0.0000
283	L	A	0.0000
284	E	A	-2.5473
285	A	A	-1.7077
286	S	A	0.0000
287	D	A	-0.8359
288	G	A	0.0000
289	I	A	0.0000
290	M	A	0.0000
291	V	A	0.0000
292	A	A	0.0000
293	R	A	-0.3528
294	G	A	-1.0150
295	D	A	-0.9272
296	L	A	0.0000
297	G	A	-0.7902
298	I	A	-0.3646
299	E	A	-0.6935
300	I	A	0.0000
301	P	A	-0.9641
302	A	A	-0.9763
303	E	A	-1.5957
304	K	A	-0.6912
305	V	A	0.3819
306	F	A	1.8440
307	L	A	1.7173
308	A	A	0.0000
309	Q	A	0.0000
310	K	A	-0.3434
311	M	A	-0.0640
312	M	A	0.0000
313	I	A	0.0000
314	G	A	-1.8180
315	R	A	-2.7107
316	C	A	0.0000
317	N	A	-1.8229
318	R	A	-3.0430
319	A	A	-1.8053
320	G	A	-1.3365
321	K	A	-1.3772
322	P	A	0.0000
323	V	A	0.0000
324	I	A	0.0000
325	C	A	0.0000
326	A	A	0.0000
327	T	A	-0.9681
328	Q	A	-1.7786
329	M	A	0.0000
330	L	A	0.0000
331	E	A	-2.0844
332	S	A	-2.0448
333	M	A	0.0000
334	I	A	-2.6754
335	K	A	-3.5156
336	K	A	-3.6297
337	P	A	-2.4862
338	R	A	-2.9506
339	P	A	-2.2668
340	T	A	-1.8319
341	R	A	-2.2419
342	A	A	-1.1359
343	E	A	-1.6874
344	G	A	-1.1830
345	S	A	-1.1163
346	D	A	-1.1868
347	V	A	0.0000
348	A	A	-0.7784
349	N	A	-1.0843
350	A	A	0.0000
351	V	A	0.0000
352	L	A	-0.2312
353	D	A	-0.7326
354	G	A	0.0000
355	A	A	0.0000
356	D	A	0.0000
357	C	A	0.0000
358	I	A	0.0000
359	M	A	0.0000
360	L	A	0.0000
361	S	A	-0.7352
362	G	A	-1.1806
363	E	A	0.0000
364	T	A	0.0000
365	A	A	-1.1447
366	K	A	-2.3100
367	G	A	0.0000
368	D	A	-2.8410
369	Y	A	-1.8055
370	P	A	0.0000
371	L	A	-0.9606
372	E	A	-1.5354
373	A	A	0.0000
374	V	A	0.0000
375	R	A	-1.3039
376	M	A	-0.5666
377	Q	A	0.0000
378	H	A	-0.4982
379	L	A	0.0765
380	I	A	-0.4113
381	A	A	0.0000
382	R	A	-0.9739
383	E	A	-1.4556
384	A	A	0.0000
385	E	A	0.0000
386	A	A	0.0000
387	A	A	-0.4326
388	M	A	0.0000
389	F	A	-0.0280
390	H	A	0.0000
391	R	A	-2.4308
392	K	A	-2.2020
393	L	A	-1.5335
394	F	A	0.0000
395	E	A	-3.4562
396	E	A	-3.6549
397	L	A	0.0000
398	V	A	-2.1072
399	R	A	-3.1921
400	A	A	-2.3919
401	S	A	-1.7893
402	S	A	-1.6842
403	H	A	-1.5769
404	S	A	-0.8974
405	T	A	-0.6286
406	D	A	-0.6488
407	L	A	0.9142
408	M	A	0.0000
409	E	A	-0.0796
410	A	A	0.2139
411	M	A	0.1400
412	A	A	0.0000
413	M	A	-0.3668
414	G	A	-0.6153
415	S	A	0.0000
416	V	A	0.0000
417	E	A	-2.3724
418	A	A	-1.0792
419	S	A	0.0000
420	Y	A	-1.1460
421	K	A	-1.6806
422	C	A	-0.1295
423	L	A	0.2463
424	A	A	0.0000
425	A	A	-0.0013
426	A	A	0.0000
427	L	A	0.0000
428	I	A	0.0000
429	V	A	0.0000
430	L	A	0.0000
431	T	A	0.0000
432	E	A	-2.5535
433	S	A	-1.9066
434	G	A	0.0000
435	R	A	-2.3043
436	S	A	0.0000
437	A	A	0.0000
438	H	A	-1.0637
439	Q	A	-0.8418
440	V	A	0.0000
441	A	A	0.0000
442	R	A	-0.7597
443	Y	A	0.0000
444	R	A	0.0000
445	P	A	-0.5055
446	R	A	-0.1414
447	A	A	0.0000
448	P	A	0.0000
449	I	A	0.0000
450	I	A	0.0000
451	A	A	0.0000
452	V	A	0.0000
453	T	A	0.0000
454	R	A	-1.9931
455	N	A	-1.8860
456	P	A	-1.3297
457	Q	A	-1.5013
458	T	A	0.0000
459	A	A	0.0000
460	R	A	-0.6848
461	Q	A	-0.6558
462	A	A	0.0000
463	H	A	0.0000
464	L	A	0.0000
465	Y	A	0.0000
466	R	A	0.0000
467	G	A	0.0000
468	I	A	0.0000
469	F	A	0.1860
470	P	A	0.0000
471	V	A	0.0000
472	L	A	-0.7980
473	C	A	0.0000
474	K	A	-2.4776
475	D	A	-2.0291
476	P	A	-0.8454
477	V	A	0.2587
478	Q	A	-1.1227
479	E	A	-1.8239
480	A	A	-1.1554
481	W	A	-1.0393
482	A	A	-1.4668
483	E	A	-2.3512
484	D	A	0.0000
485	V	A	-1.1154
486	D	A	-2.3776
487	L	A	-1.2525
488	R	A	0.0000
489	V	A	0.0000
490	N	A	-1.5164
491	F	A	-0.8741
492	A	A	0.0000
493	M	A	0.0000
494	N	A	-1.4850
495	V	A	0.0000
496	G	A	0.0000
497	K	A	-1.8989
498	A	A	-0.6893
499	R	A	-0.4769
500	G	A	-0.9570
501	F	A	-0.5608
502	F	A	0.0000
503	K	A	-3.2120
504	K	A	-3.1725
505	G	A	-2.1041
506	D	A	-1.7680
507	V	A	-0.1324
508	V	A	0.0000
509	I	A	0.0000
510	V	A	0.0000
511	L	A	0.0000
512	T	A	0.0000
513	G	A	-0.2707
514	W	A	0.0988
515	R	A	-1.5926
516	P	A	-1.0979
517	G	A	-1.1349
518	S	A	-0.9344
519	G	A	-1.1105
520	F	A	-0.5679
521	T	A	0.0000
522	N	A	-0.0659
523	T	A	-0.0603
524	M	A	0.3604
525	R	A	0.1011
526	V	A	1.2778
527	V	A	0.5716
528	P	A	-0.6922
529	V	A	0.0000
530	P	A	-1.4780