Project name: SH3_F87N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	FA87N
Energy difference between WT (input) and mutated protein (by FoldX)	4.8055 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7178
• Average score: -0.9844
• Total score value: -56.1119

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4676
86	L	A	0.6878
87	N	A	0.0000	mutated: FA87N
88	V	A	0.3187
89	A	A	0.0000
90	L	A	-0.1781
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4855
107	E	A	-1.3428
108	K	A	-0.7113
109	F	A	0.0000
110	Q	A	-0.5447
111	I	A	-0.0583
112	L	A	0.1688
113	N	A	-0.8620
114	S	A	-1.1519
115	S	A	-1.5866
116	E	A	-2.5491
117	G	A	-2.1227
118	D	A	-2.4296
119	W	A	-1.0762
120	W	A	-0.9955
121	E	A	-1.1183
122	A	A	0.0000
123	R	A	-1.7023
124	S	A	0.0000
125	L	A	0.0353
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6891
131	G	A	-1.4872
132	Y	A	-0.8460
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9302
136	N	A	-1.1444
137	Y	A	-0.1505
138	V	A	0.0000
139	A	A	0.3385
140	P	A	0.6764
141	V	A	1.7178