Project name: SH3_W119G

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA119G
Energy difference between WT (input) and mutated protein (by FoldX)	3.21768 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.0947
• Maximal score value: 1.7964
• Average score: -1.019
• Total score value: -58.0842

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9240
88	V	A	0.4316
89	A	A	0.0000
90	L	A	-0.1470
91	Y	A	-0.5669
92	D	A	-2.5455
93	Y	A	-1.9081
94	E	A	-2.6312
95	A	A	-2.6144
96	R	A	-2.9819
97	T	A	-2.6608
98	E	A	-3.0947
99	D	A	-3.0254
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1883
103	F	A	0.0000
104	H	A	-2.7210
105	K	A	-2.4006
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5093
111	I	A	-0.0500
112	L	A	0.1492
113	N	A	-0.9767
114	S	A	-1.3149
115	S	A	-1.6840
116	E	A	-2.6907
117	G	A	-2.3371
118	D	A	-2.7742
119	G	A	-1.7649	mutated: WA119G
120	W	A	-1.3762
121	E	A	-1.2875
122	A	A	0.0000
123	R	A	-1.7063
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.6789
131	G	A	-1.5450
132	Y	A	-1.0109
133	I	A	0.0000
134	P	A	-0.8438
135	S	A	-1.1469
136	N	A	-1.2629
137	Y	A	-0.2014
138	V	A	0.0000
139	A	A	0.4162
140	P	A	0.7757
141	V	A	1.7964