Project name: SH3_G131T

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA131T
Energy difference between WT (input) and mutated protein (by FoldX)	6.41481 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.0912
• Maximal score value: 1.7964
• Average score: -0.9534
• Total score value: -54.3449

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9247
88	V	A	0.4331
89	A	A	0.0000
90	L	A	-0.1503
91	Y	A	-0.5741
92	D	A	-2.5587
93	Y	A	-1.9307
94	E	A	-2.6441
95	A	A	-2.6155
96	R	A	-2.9846
97	T	A	-2.6605
98	E	A	-3.0912
99	D	A	-3.0199
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1923
103	F	A	0.0000
104	H	A	-2.7260
105	K	A	-2.4041
106	G	A	-1.4553
107	E	A	-1.3021
108	K	A	-0.6399
109	F	A	0.0000
110	Q	A	-0.5082
111	I	A	-0.0484
112	L	A	0.1516
113	N	A	-0.8863
114	S	A	-1.1774
115	S	A	-1.5973
116	E	A	-2.5609
117	G	A	-2.1350
118	D	A	-2.4419
119	W	A	-1.0903
120	W	A	-1.0391
121	E	A	-1.1241
122	A	A	0.0000
123	R	A	-1.6968
124	S	A	0.0000
125	L	A	0.0615
126	T	A	-0.4716
127	T	A	-0.8186
128	G	A	-1.3504
129	E	A	-2.2253
130	T	A	-1.6548
131	T	A	-1.4355	mutated: GA131T
132	Y	A	-0.8109
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9167
136	N	A	-1.1508
137	Y	A	-0.1246
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964