Project name: SH3_T127P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	TA127P
Energy difference between WT (input) and mutated protein (by FoldX)	2.58835 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.103
• Maximal score value: 1.7964
• Average score: -0.97
• Total score value: -55.2926

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7939
87	F	A	0.9239
88	V	A	0.4309
89	A	A	0.0000
90	L	A	-0.1517
91	Y	A	-0.5752
92	D	A	-2.5606
93	Y	A	-1.9320
94	E	A	-2.6458
95	A	A	-2.6265
96	R	A	-2.9854
97	T	A	-2.6610
98	E	A	-3.1030
99	D	A	-3.0436
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2075
103	F	A	0.0000
104	H	A	-2.7296
105	K	A	-2.4070
106	G	A	-1.4598
107	E	A	-1.3313
108	K	A	-0.6613
109	F	A	0.0000
110	Q	A	-0.5079
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8883
114	S	A	-1.1799
115	S	A	-1.5966
116	E	A	-2.5603
117	G	A	-2.1347
118	D	A	-2.4456
119	W	A	-1.1053
120	W	A	-1.0569
121	E	A	-1.1536
122	A	A	0.0000
123	R	A	-1.7435
124	S	A	0.0000
125	L	A	0.0110
126	T	A	-0.5598
127	P	A	-1.0034	mutated: TA127P
128	G	A	-1.4456
129	E	A	-2.2989
130	T	A	-1.7172
131	G	A	-1.4997
132	Y	A	-0.8683
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9214
136	N	A	-1.1508
137	Y	A	-0.1246
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964