Project name: SH3_L86E

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA86E
Energy difference between WT (input) and mutated protein (by FoldX)	1.23378 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.4092
• Average score: -1.0732
• Total score value: -61.1698

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	-0.1947
86	E	A	-0.6494	mutated: LA86E
87	F	A	0.1588
88	V	A	0.0422
89	A	A	0.0000
90	L	A	-0.1132
91	Y	A	-0.5342
92	D	A	-2.5196
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0436
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2061
103	F	A	0.0000
104	H	A	-2.6765
105	K	A	-2.3020
106	G	A	-1.2388
107	E	A	-1.2569
108	K	A	-1.1062
109	F	A	0.0000
110	Q	A	-1.4782
111	I	A	-0.5343
112	L	A	0.0978
113	N	A	-0.9142
114	S	A	-1.1623
115	S	A	-1.5884
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0853
121	E	A	-1.1841
122	A	A	0.0000
123	R	A	-1.9486
124	S	A	0.0000
125	L	A	-0.2930
126	T	A	-0.4878
127	T	A	-0.8386
128	G	A	-1.4182
129	E	A	-2.2285
130	T	A	-1.7203
131	G	A	-1.4731
132	Y	A	-0.8480
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9195
136	N	A	-1.1504
137	Y	A	-0.1371
138	V	A	0.0000
139	A	A	0.2249
140	P	A	0.3409
141	V	A	1.4092