Project name: SH3_I111P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	IA111P
Energy difference between WT (input) and mutated protein (by FoldX)	2.21626 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.796
• Average score: -0.9955
• Total score value: -56.7439

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4400
86	L	A	0.6933
87	F	A	0.8351
88	V	A	0.4307
89	A	A	0.0000
90	L	A	-0.1513
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4582
107	E	A	-1.3080
108	K	A	-0.6917
109	F	A	0.0000
110	Q	A	-0.7272
111	P	A	-0.5061	mutated: IA111P
112	L	A	-0.0680
113	N	A	-1.0228
114	S	A	-1.3040
115	S	A	-1.6088
116	E	A	-2.5687
117	G	A	-2.1415
118	D	A	-2.4508
119	W	A	-1.1086
120	W	A	-1.1649
121	E	A	-1.2740
122	A	A	0.0000
123	R	A	-1.8216
124	S	A	0.0000
125	L	A	0.0590
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.7430
131	G	A	-1.5424
132	Y	A	-0.9180
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9736
136	N	A	-1.1503
137	Y	A	-0.1245
138	V	A	0.0000
139	A	A	0.4154
140	P	A	0.7751
141	V	A	1.7960