Project name: SH3_F87P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	FA87P
Energy difference between WT (input) and mutated protein (by FoldX)	6.28434 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7167
• Average score: -0.983
• Total score value: -56.0336

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4689
86	L	A	0.6883
87	P	A	0.0000	mutated: FA87P
88	V	A	0.3150
89	A	A	0.0000
90	L	A	-0.1789
91	Y	A	-0.5750
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7289
105	K	A	-2.4069
106	G	A	-1.4872
107	E	A	-1.3455
108	K	A	-0.7153
109	F	A	0.0000
110	Q	A	-0.5403
111	I	A	-0.0471
112	L	A	0.1758
113	N	A	-0.8553
114	S	A	-1.1449
115	S	A	-1.5842
116	E	A	-2.5464
117	G	A	-2.1199
118	D	A	-2.4259
119	W	A	-1.0696
120	W	A	-0.9803
121	E	A	-1.1095
122	A	A	0.0000
123	R	A	-1.6994
124	S	A	0.0000
125	L	A	0.0337
126	T	A	-0.4739
127	T	A	-0.8211
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6881
131	G	A	-1.4841
132	Y	A	-0.8406
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9254
136	N	A	-1.1429
137	Y	A	-0.1508
138	V	A	0.0000
139	A	A	0.3378
140	P	A	0.6755
141	V	A	1.7167