Project name: SH3_S114V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	SA114V
Energy difference between WT (input) and mutated protein (by FoldX)	1.432 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1027
• Maximal score value: 1.7956
• Average score: -0.8551
• Total score value: -48.739

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5067
86	L	A	0.7791
87	F	A	0.9100
88	V	A	0.4301
89	A	A	0.0000
90	L	A	-0.1514
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6257
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1027
99	D	A	-3.0431
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2058
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4584
107	E	A	-1.3082
108	K	A	-0.6497
109	F	A	0.0000
110	Q	A	-0.5428
111	I	A	0.2019
112	L	A	0.4393
113	N	A	-0.1994
114	V	A	0.2860	mutated: SA114V
115	S	A	-0.8736
116	E	A	-2.1148
117	G	A	-1.7557
118	D	A	-2.1195
119	W	A	-0.8025
120	W	A	-0.5190
121	E	A	-0.8410
122	A	A	0.0000
123	R	A	-1.7332
124	S	A	0.0000
125	L	A	0.0589
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6998
131	G	A	-1.5098
132	Y	A	-0.7249
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.6940
136	N	A	-1.1538
137	Y	A	-0.1249
138	V	A	0.0000
139	A	A	0.4150
140	P	A	0.7746
141	V	A	1.7956