Project name: SH3_L112G

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA112G
Energy difference between WT (input) and mutated protein (by FoldX)	2.17066 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1025
• Maximal score value: 1.7964
• Average score: -1.1237
• Total score value: -64.0531

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4644
86	L	A	0.6343
87	F	A	0.8517
88	V	A	0.3852
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5597
93	Y	A	-1.9312
94	E	A	-2.6448
95	A	A	-2.6253
96	R	A	-2.9850
97	T	A	-2.6607
98	E	A	-3.1025
99	D	A	-3.0617
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2030
103	F	A	0.0000
104	H	A	-2.7280
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3252
108	K	A	-0.8034
109	F	A	0.0000
110	Q	A	-1.3059
111	I	A	-0.9312
112	G	A	-1.2928	mutated: LA112G
113	N	A	-1.5714
114	S	A	-1.4877
115	S	A	-1.7623
116	E	A	-2.5835
117	G	A	-2.1516
118	D	A	-2.4635
119	W	A	-1.1423
120	W	A	-1.4618
121	E	A	-1.8571
122	A	A	0.0000
123	R	A	-2.6592
124	S	A	0.0000
125	L	A	-0.2618
126	T	A	-0.6293
127	T	A	-1.0023
128	G	A	-1.7282
129	E	A	-2.4834
130	T	A	-2.1869
131	G	A	-1.8167
132	Y	A	-1.1360
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9374
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7465
141	V	A	1.7964