Project name: SH3_W120P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA120P
Energy difference between WT (input) and mutated protein (by FoldX)	4.20985 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8562
• Average score: -0.9461
• Total score value: -53.9261

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5725
86	L	A	0.8956
87	F	A	1.1065
88	V	A	0.5168
89	A	A	0.0000
90	L	A	-0.1305
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4373
107	E	A	-1.2814
108	K	A	-0.5796
109	F	A	0.0000
110	Q	A	-0.4450
111	I	A	-0.0039
112	L	A	0.1307
113	N	A	-0.9673
114	S	A	-1.3312
115	S	A	-1.6900
116	E	A	-2.6587
117	G	A	-2.2799
118	D	A	-2.5663
119	W	A	-1.2480
120	P	A	0.0000	mutated: WA120P
121	E	A	-1.2490
122	A	A	0.0000
123	R	A	-1.7145
124	S	A	0.0000
125	L	A	0.0772
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6835
131	G	A	-1.5205
132	Y	A	-0.9318
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-1.0101
136	N	A	-1.1919
137	Y	A	-0.1198
138	V	A	0.0000
139	A	A	0.4678
140	P	A	0.8513
141	V	A	1.8562