| Chain sequence(s) |
A: HHSSGLEVLFQGPGSDAPEEEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLPDGAAAESLVESSEVAVIGFFKDVESDSAKQFLQAAEAIDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGRNNFEGEVTKENLLDFIKHNQLPLVIEAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFCAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGA |
| Distance of aggregation | 10 Å |
| Dynamic mode | No |
| Mutated residues | LA6V |
| Energy difference between WT (input) and mutated protein (by FoldX) | 0.333291 kcal/mol
Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive. |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 1 | H | A | -1.6343 | |
| 2 | H | A | -1.6142 | |
| 3 | S | A | -0.8803 | |
| 4 | S | A | -0.8898 | |
| 5 | G | A | -0.5446 | |
| 6 | V | A | 0.6932 | mutated: LA6V |
| 7 | E | A | 0.0904 | |
| 8 | V | A | 2.1611 | |
| 9 | L | A | 2.5417 | |
| 10 | F | A | 1.9151 | |
| 11 | Q | A | -0.1339 | |
| 12 | G | A | -0.9227 | |
| 13 | P | A | -1.0588 | |
| 14 | G | A | -1.1423 | |
| 15 | S | A | -1.9546 | |
| 16 | D | A | -2.3824 | |
| 17 | A | A | -1.8684 | |
| 18 | P | A | -2.3835 | |
| 19 | E | A | -3.3209 | |
| 20 | E | A | -3.7747 | |
| 21 | E | A | -3.2611 | |
| 22 | D | A | -3.2702 | |
| 23 | H | A | -3.2024 | |
| 24 | V | A | 0.0000 | |
| 25 | L | A | 0.0000 | |
| 26 | V | A | -1.5112 | |
| 27 | L | A | 0.0000 | |
| 28 | R | A | -1.8799 | |
| 29 | K | A | -2.8025 | |
| 30 | S | A | -1.9083 | |
| 31 | N | A | -1.9198 | |
| 32 | F | A | 0.0000 | |
| 33 | A | A | -1.4596 | |
| 34 | E | A | -1.8648 | |
| 35 | A | A | 0.0000 | |
| 36 | L | A | 0.0000 | |
| 37 | A | A | -1.6404 | |
| 38 | A | A | -1.0005 | |
| 39 | H | A | -1.7995 | |
| 40 | K | A | -2.6850 | |
| 41 | Y | A | -1.6186 | |
| 42 | L | A | 0.0000 | |
| 43 | L | A | 0.0000 | |
| 44 | V | A | 0.0000 | |
| 45 | E | A | 0.0000 | |
| 46 | F | A | 0.0000 | |
| 47 | Y | A | -0.2299 | |
| 48 | A | A | 0.0000 | |
| 49 | P | A | -0.1217 | |
| 50 | W | A | 0.6367 | |
| 51 | C | A | -0.2924 | |
| 52 | G | A | -1.1581 | |
| 53 | H | A | -1.6550 | |
| 54 | C | A | 0.0000 | |
| 55 | K | A | -1.9425 | |
| 56 | A | A | -1.5269 | |
| 57 | L | A | 0.0000 | |
| 58 | A | A | -1.4285 | |
| 59 | P | A | -1.5579 | |
| 60 | E | A | -2.2744 | |
| 61 | Y | A | 0.0000 | |
| 62 | A | A | -1.9723 | |
| 63 | K | A | -3.1539 | |
| 64 | A | A | 0.0000 | |
| 65 | A | A | 0.0000 | |
| 66 | G | A | -2.6902 | |
| 67 | K | A | -2.7949 | |
| 68 | L | A | 0.0000 | |
| 69 | K | A | -3.4718 | |
| 70 | A | A | -2.1944 | |
| 71 | E | A | -3.1120 | |
| 72 | G | A | -2.5453 | |
| 73 | S | A | -2.4250 | |
| 74 | E | A | -2.7748 | |
| 75 | I | A | 0.0000 | |
| 76 | R | A | -1.4571 | |
| 77 | L | A | 0.0000 | |
| 78 | A | A | 0.0000 | |
| 79 | K | A | -0.5816 | |
| 80 | V | A | 0.0000 | |
| 81 | D | A | -1.0836 | |
| 82 | A | A | 0.0000 | |
| 83 | T | A | -1.2651 | |
| 84 | E | A | -2.6091 | |
| 85 | E | A | -2.5590 | |
| 86 | S | A | -2.5608 | |
| 87 | D | A | -3.3004 | |
| 88 | L | A | 0.0000 | |
| 89 | A | A | 0.0000 | |
| 90 | Q | A | -2.9934 | |
| 91 | Q | A | -2.3913 | |
| 92 | Y | A | -1.5800 | |
| 93 | G | A | -1.9585 | |
| 94 | V | A | 0.0000 | |
| 95 | R | A | -2.3198 | |
| 96 | G | A | -1.2265 | |
| 97 | Y | A | -0.3886 | |
| 98 | P | A | -0.6259 | |
| 99 | T | A | -0.7324 | |
| 100 | I | A | 0.0000 | |
| 101 | K | A | -1.1433 | |
| 102 | F | A | 0.0000 | |
| 103 | F | A | 0.0000 | |
| 104 | R | A | -2.0898 | |
| 105 | N | A | -2.6617 | |
| 106 | G | A | -2.3089 | |
| 107 | D | A | -2.4904 | |
| 108 | T | A | -1.1537 | |
| 109 | A | A | -0.7077 | |
| 110 | S | A | -1.2120 | |
| 111 | P | A | -1.3048 | |
| 112 | K | A | -1.8035 | |
| 113 | E | A | -2.2180 | |
| 114 | Y | A | 0.0000 | |
| 115 | T | A | -0.9066 | |
| 116 | A | A | -1.2125 | |
| 117 | G | A | -2.1803 | |
| 118 | R | A | -3.4056 | |
| 119 | E | A | -3.8782 | |
| 120 | A | A | 0.0000 | |
| 121 | D | A | -3.2958 | |
| 122 | D | A | -3.0733 | |
| 123 | I | A | 0.0000 | |
| 124 | V | A | 0.0000 | |
| 125 | N | A | -2.1943 | |
| 126 | W | A | -1.7231 | |
| 127 | L | A | 0.0000 | |
| 128 | K | A | -2.6523 | |
| 129 | K | A | -2.9623 | |
| 130 | R | A | -2.3069 | |
| 131 | T | A | -1.9619 | |
| 132 | G | A | -1.3153 | |
| 133 | P | A | -0.9637 | |
| 134 | A | A | -1.1000 | |
| 135 | A | A | 0.0000 | |
| 136 | T | A | -0.1756 | |
| 137 | T | A | -0.3065 | |
| 138 | L | A | 0.0000 | |
| 139 | P | A | -1.1681 | |
| 140 | D | A | -2.0473 | |
| 141 | G | A | 0.0000 | |
| 142 | A | A | -0.7013 | |
| 143 | A | A | -0.9158 | |
| 144 | A | A | 0.0000 | |
| 145 | E | A | -1.8051 | |
| 146 | S | A | -1.2366 | |
| 147 | L | A | -0.8652 | |
| 148 | V | A | -1.5910 | |
| 149 | E | A | -2.7048 | |
| 150 | S | A | -1.7435 | |
| 151 | S | A | -2.0014 | |
| 152 | E | A | -2.7370 | |
| 153 | V | A | 0.0000 | |
| 154 | A | A | 0.0000 | |
| 155 | V | A | 0.0000 | |
| 156 | I | A | 0.0000 | |
| 157 | G | A | 0.0000 | |
| 158 | F | A | 0.0000 | |
| 159 | F | A | 0.0000 | |
| 160 | K | A | -3.2035 | |
| 161 | D | A | -3.1409 | |
| 162 | V | A | -1.8830 | |
| 163 | E | A | -3.0013 | |
| 164 | S | A | -2.8979 | |
| 165 | D | A | -3.0263 | |
| 166 | S | A | -2.4745 | |
| 167 | A | A | 0.0000 | |
| 168 | K | A | -2.9967 | |
| 169 | Q | A | -2.5053 | |
| 170 | F | A | 0.0000 | |
| 171 | L | A | -1.3051 | |
| 172 | Q | A | -1.6300 | |
| 173 | A | A | 0.0000 | |
| 174 | A | A | 0.0000 | |
| 175 | E | A | -1.2999 | |
| 176 | A | A | -0.9303 | |
| 177 | I | A | -1.3681 | |
| 178 | D | A | -2.9453 | |
| 179 | D | A | -2.5942 | |
| 180 | I | A | 0.0000 | |
| 181 | P | A | -0.9380 | |
| 182 | F | A | 0.0000 | |
| 183 | G | A | 0.0000 | |
| 184 | I | A | 0.0000 | |
| 185 | T | A | 0.0000 | |
| 186 | S | A | -1.8716 | |
| 187 | N | A | -1.9842 | |
| 188 | S | A | -2.1885 | |
| 189 | D | A | -2.8613 | |
| 190 | V | A | 0.0000 | |
| 191 | F | A | 0.0000 | |
| 192 | S | A | -2.3571 | |
| 193 | K | A | -2.3195 | |
| 194 | Y | A | 0.0000 | |
| 195 | Q | A | -2.2898 | |
| 196 | L | A | -2.2743 | |
| 197 | D | A | -2.9469 | |
| 198 | K | A | -3.2961 | |
| 199 | D | A | -2.5314 | |
| 200 | G | A | -1.9683 | |
| 201 | V | A | 0.0000 | |
| 202 | V | A | 0.0000 | |
| 203 | L | A | 0.0000 | |
| 204 | F | A | 0.0000 | |
| 205 | K | A | 0.0000 | |
| 206 | K | A | -3.1774 | |
| 207 | F | A | -2.1482 | |
| 208 | D | A | -2.8862 | |
| 209 | E | A | -3.0860 | |
| 210 | G | A | -2.3119 | |
| 211 | R | A | -2.0467 | |
| 212 | N | A | -1.6231 | |
| 213 | N | A | -1.7890 | |
| 214 | F | A | -1.7161 | |
| 215 | E | A | -2.7084 | |
| 216 | G | A | -2.2764 | |
| 217 | E | A | -2.7311 | |
| 218 | V | A | -2.2986 | |
| 219 | T | A | -2.4115 | |
| 220 | K | A | -2.6071 | |
| 221 | E | A | -3.0876 | |
| 222 | N | A | -2.8935 | |
| 223 | L | A | 0.0000 | |
| 224 | L | A | -1.8809 | |
| 225 | D | A | -2.9597 | |
| 226 | F | A | -2.1713 | |
| 227 | I | A | 0.0000 | |
| 228 | K | A | -2.1606 | |
| 229 | H | A | -2.4083 | |
| 230 | N | A | -1.4390 | |
| 231 | Q | A | -0.9847 | |
| 232 | L | A | -0.4883 | |
| 233 | P | A | -0.7863 | |
| 234 | L | A | -1.0131 | |
| 235 | V | A | -0.6108 | |
| 236 | I | A | 0.0000 | |
| 237 | E | A | -1.5793 | |
| 238 | A | A | -0.1251 | |
| 239 | P | A | 0.0034 | |
| 240 | K | A | -0.4969 | |
| 241 | I | A | 0.1567 | |
| 242 | F | A | 1.1730 | |
| 243 | G | A | -0.2877 | |
| 244 | G | A | -1.0628 | |
| 245 | E | A | -2.1295 | |
| 246 | I | A | -1.5691 | |
| 247 | K | A | -1.7819 | |
| 248 | T | A | -1.2789 | |
| 249 | H | A | -0.4156 | |
| 250 | I | A | 0.0000 | |
| 251 | L | A | -0.0310 | |
| 252 | L | A | 0.0000 | |
| 253 | F | A | 0.0000 | |
| 254 | L | A | 0.0000 | |
| 255 | P | A | -1.8616 | |
| 256 | K | A | -2.6360 | |
| 257 | S | A | -1.5626 | |
| 258 | V | A | -1.3030 | |
| 259 | S | A | -1.5167 | |
| 260 | D | A | -2.6811 | |
| 261 | Y | A | -2.1845 | |
| 262 | D | A | -2.8472 | |
| 263 | G | A | -2.1050 | |
| 264 | K | A | -1.8193 | |
| 265 | L | A | -1.4915 | |
| 266 | S | A | -1.6262 | |
| 267 | N | A | -1.6592 | |
| 268 | F | A | 0.0000 | |
| 269 | K | A | -1.9211 | |
| 270 | T | A | -1.6249 | |
| 271 | A | A | 0.0000 | |
| 272 | A | A | 0.0000 | |
| 273 | E | A | -2.5505 | |
| 274 | S | A | -1.9714 | |
| 275 | F | A | 0.0000 | |
| 276 | K | A | -2.8529 | |
| 277 | G | A | -2.3789 | |
| 278 | K | A | -2.6558 | |
| 279 | I | A | 0.0000 | |
| 280 | L | A | -0.8404 | |
| 281 | F | A | 0.0000 | |
| 282 | I | A | 0.0000 | |
| 283 | F | A | -0.3559 | |
| 284 | I | A | -0.8024 | |
| 285 | D | A | -1.9834 | |
| 286 | S | A | 0.0000 | |
| 287 | D | A | -3.2389 | |
| 288 | H | A | -2.8770 | |
| 289 | T | A | -2.4930 | |
| 290 | D | A | -3.0279 | |
| 291 | N | A | -2.7147 | |
| 292 | Q | A | -3.5816 | |
| 293 | R | A | -2.8774 | |
| 294 | I | A | -1.3923 | |
| 295 | L | A | 0.0000 | |
| 296 | E | A | -2.9063 | |
| 297 | F | A | -0.9664 | |
| 298 | F | A | 0.0000 | |
| 299 | G | A | -1.7203 | |
| 300 | L | A | 0.0000 | |
| 301 | K | A | -3.8180 | |
| 302 | K | A | -3.8160 | |
| 303 | E | A | -3.7787 | |
| 304 | E | A | -3.5244 | |
| 305 | C | A | -2.1977 | |
| 306 | P | A | -1.0754 | |
| 307 | A | A | -1.1963 | |
| 308 | V | A | 0.0000 | |
| 309 | R | A | -0.4159 | |
| 310 | L | A | 0.0000 | |
| 311 | I | A | 0.0000 | |
| 312 | T | A | -0.6701 | |
| 313 | L | A | -0.7897 | |
| 314 | E | A | -1.9668 | |
| 315 | M | A | -0.7696 | |
| 316 | T | A | -0.7708 | |
| 317 | K | A | -0.3815 | |
| 318 | Y | A | -0.8851 | |
| 319 | K | A | -1.5197 | |
| 320 | P | A | -2.3487 | |
| 321 | E | A | -3.0745 | |
| 322 | S | A | -2.5281 | |
| 323 | E | A | -3.1063 | |
| 324 | E | A | -2.9207 | |
| 325 | L | A | -1.5608 | |
| 326 | T | A | -1.7947 | |
| 327 | A | A | 0.0000 | |
| 328 | E | A | -2.8754 | |
| 329 | R | A | -3.2754 | |
| 330 | I | A | 0.0000 | |
| 331 | T | A | -1.9852 | |
| 332 | E | A | -2.3765 | |
| 333 | F | A | 0.0000 | |
| 334 | C | A | 0.0000 | |
| 335 | H | A | -1.5470 | |
| 336 | R | A | -2.1826 | |
| 337 | F | A | -1.3882 | |
| 338 | L | A | -0.8769 | |
| 339 | E | A | -2.3143 | |
| 340 | G | A | -2.2798 | |
| 341 | K | A | -2.9079 | |
| 342 | I | A | 0.0000 | |
| 343 | K | A | -2.5181 | |
| 344 | P | A | -1.4601 | |
| 345 | H | A | -0.7786 | |
| 346 | L | A | -0.4634 | |
| 347 | M | A | -0.6315 | |
| 348 | S | A | -1.3501 | |
| 349 | Q | A | -2.0707 | |
| 350 | E | A | -2.6422 | |
| 351 | L | A | -1.8153 | |
| 352 | P | A | -2.0744 | |
| 353 | E | A | -3.3237 | |
| 354 | D | A | -3.8051 | |
| 355 | W | A | -2.6069 | |
| 356 | D | A | -3.4167 | |
| 357 | K | A | -3.8385 | |
| 358 | Q | A | -3.4094 | |
| 359 | P | A | -3.0410 | |
| 360 | V | A | 0.0000 | |
| 361 | K | A | -1.7739 | |
| 362 | V | A | -0.8875 | |
| 363 | L | A | 0.0000 | |
| 364 | V | A | 0.0000 | |
| 365 | G | A | -1.8358 | |
| 366 | K | A | -2.5032 | |
| 367 | N | A | 0.0000 | |
| 368 | F | A | 0.0000 | |
| 369 | E | A | -2.0847 | |
| 370 | D | A | -2.5473 | |
| 371 | V | A | -1.6152 | |
| 372 | A | A | 0.0000 | |
| 373 | F | A | -2.1929 | |
| 374 | D | A | -2.9974 | |
| 375 | E | A | -3.9607 | |
| 376 | K | A | -3.5084 | |
| 377 | K | A | -2.4665 | |
| 378 | N | A | -1.5021 | |
| 379 | V | A | 0.0000 | |
| 380 | F | A | 0.0000 | |
| 381 | V | A | 0.0000 | |
| 382 | E | A | 0.0000 | |
| 383 | F | A | 0.0000 | |
| 384 | C | A | -0.4232 | |
| 385 | A | A | 0.0000 | |
| 386 | P | A | -0.1983 | |
| 387 | W | A | -0.1114 | |
| 388 | C | A | -0.7277 | |
| 389 | G | A | -1.6677 | |
| 390 | H | A | -2.0384 | |
| 391 | C | A | 0.0000 | |
| 392 | K | A | -2.6800 | |
| 393 | Q | A | -2.4910 | |
| 394 | L | A | 0.0000 | |
| 395 | A | A | -1.5923 | |
| 396 | P | A | -1.3610 | |
| 397 | I | A | -1.3389 | |
| 398 | W | A | 0.0000 | |
| 399 | D | A | -2.4668 | |
| 400 | K | A | -2.3952 | |
| 401 | L | A | 0.0000 | |
| 402 | G | A | 0.0000 | |
| 403 | E | A | -3.5730 | |
| 404 | T | A | -2.3257 | |
| 405 | Y | A | 0.0000 | |
| 406 | K | A | -3.8135 | |
| 407 | D | A | -3.6749 | |
| 408 | H | A | -2.9919 | |
| 409 | E | A | -3.3034 | |
| 410 | N | A | -2.6053 | |
| 411 | I | A | -1.8111 | |
| 412 | V | A | -0.9177 | |
| 413 | I | A | 0.0000 | |
| 414 | A | A | 0.0000 | |
| 415 | K | A | -0.6820 | |
| 416 | M | A | 0.0000 | |
| 417 | D | A | -0.5606 | |
| 418 | S | A | 0.0000 | |
| 419 | T | A | 0.0000 | |
| 420 | A | A | -0.6932 | |
| 421 | N | A | 0.0000 | |
| 422 | E | A | -0.7818 | |
| 423 | V | A | 0.0000 | |
| 424 | E | A | -1.9290 | |
| 425 | A | A | -1.1799 | |
| 426 | V | A | 0.0000 | |
| 427 | K | A | -1.0713 | |
| 428 | V | A | -0.3347 | |
| 429 | H | A | -1.0762 | |
| 430 | S | A | -0.4312 | |
| 431 | F | A | -0.1799 | |
| 432 | P | A | 0.0000 | |
| 433 | T | A | -0.6156 | |
| 434 | L | A | 0.0000 | |
| 435 | K | A | -0.9021 | |
| 436 | F | A | 0.0000 | |
| 437 | F | A | 0.0000 | |
| 438 | P | A | -1.3965 | |
| 439 | A | A | -2.3915 | |
| 440 | S | A | -2.2733 | |
| 441 | A | A | -2.0174 | |
| 442 | D | A | -3.2921 | |
| 443 | R | A | -2.8044 | |
| 444 | T | A | -1.0094 | |
| 445 | V | A | -0.2426 | |
| 446 | I | A | -0.7448 | |
| 447 | D | A | -1.9161 | |
| 448 | Y | A | 0.0000 | |
| 449 | N | A | -2.4397 | |
| 450 | G | A | -2.5065 | |
| 451 | E | A | -3.0860 | |
| 452 | R | A | -2.5136 | |
| 453 | T | A | -1.8109 | |
| 454 | L | A | -1.7826 | |
| 455 | D | A | -2.8136 | |
| 456 | G | A | -2.9325 | |
| 457 | F | A | 0.0000 | |
| 458 | K | A | -2.6380 | |
| 459 | K | A | -3.6907 | |
| 460 | F | A | 0.0000 | |
| 461 | L | A | 0.0000 | |
| 462 | E | A | -3.1096 | |
| 463 | S | A | -2.6070 | |
| 464 | G | A | -2.0336 | |
| 465 | G | A | -2.2707 | |
| 466 | Q | A | -2.6593 | |
| 467 | D | A | -3.2560 | |
| 468 | G | A | -2.9489 | |
| 469 | A | A | -2.1591 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine