Project name: SH3_L112V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA112V
Energy difference between WT (input) and mutated protein (by FoldX)	1.14092 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1029
• Maximal score value: 1.7964
• Average score: -0.9421
• Total score value: -53.6974

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4592
86	L	A	0.6439
87	F	A	0.8395
88	V	A	0.3770
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5597
93	Y	A	-1.9312
94	E	A	-2.6448
95	A	A	-2.6256
96	R	A	-2.9850
97	T	A	-2.6607
98	E	A	-3.1029
99	D	A	-3.0446
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2042
103	F	A	0.0000
104	H	A	-2.7280
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3372
108	K	A	-0.7901
109	F	A	0.0000
110	Q	A	-0.6641
111	I	A	0.2502
112	V	A	1.0452	mutated: LA112V
113	N	A	-0.4086
114	S	A	-0.9398
115	S	A	-1.4717
116	E	A	-2.5573
117	G	A	-2.1326
118	D	A	-2.4434
119	W	A	-1.1019
120	W	A	-0.9034
121	E	A	-0.9105
122	A	A	0.0000
123	R	A	-1.9046
124	S	A	0.0000
125	L	A	-0.2042
126	T	A	-0.5724
127	T	A	-0.8883
128	G	A	-1.4849
129	E	A	-2.3276
130	T	A	-1.7216
131	G	A	-1.4433
132	Y	A	-0.7921
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9187
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7450
141	V	A	1.7964