Project name: SH3_G106H

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA106H
Energy difference between WT (input) and mutated protein (by FoldX)	3.44864 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7281
• Average score: -0.9435
• Total score value: -53.7818

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5112
86	L	A	0.7822
87	F	A	0.8346
88	V	A	0.3599
89	A	A	0.0000
90	L	A	-0.0325
91	Y	A	-0.3452
92	D	A	-2.3388
93	Y	A	-1.7980
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.4947
105	K	A	-1.9022
106	H	A	-1.6184	mutated: GA106H
107	E	A	-1.3331
108	K	A	-0.6855
109	F	A	0.0000
110	Q	A	-0.5138
111	I	A	-0.0560
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7128
124	S	A	0.0000
125	L	A	0.0613
126	T	A	-0.5222
127	T	A	-0.8180
128	G	A	-1.3554
129	E	A	-2.2392
130	T	A	-1.6920
131	G	A	-1.4992
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.0305
138	V	A	0.0000
139	A	A	0.3452
140	P	A	0.7611
141	V	A	1.7281