Project name: SH3_L112T

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA112T
Energy difference between WT (input) and mutated protein (by FoldX)	2.28293 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1029
• Maximal score value: 1.7949
• Average score: -1.07
• Total score value: -60.9875

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4513
86	L	A	0.6086
87	F	A	0.8175
88	V	A	0.3571
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5597
93	Y	A	-1.9312
94	E	A	-2.6448
95	A	A	-2.6256
96	R	A	-2.9850
97	T	A	-2.6607
98	E	A	-3.1029
99	D	A	-3.0422
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2044
103	F	A	0.0000
104	H	A	-2.7280
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3628
108	K	A	-0.8436
109	F	A	0.0000
110	Q	A	-1.1863
111	I	A	-0.6563
112	T	A	-0.7952	mutated: LA112T
113	N	A	-1.2882
114	S	A	-1.3414
115	S	A	-1.6854
116	E	A	-2.5516
117	G	A	-2.1288
118	D	A	-2.4397
119	W	A	-1.0952
120	W	A	-1.2647
121	E	A	-1.4997
122	A	A	0.0000
123	R	A	-2.3442
124	S	A	0.0000
125	L	A	-0.3444
126	T	A	-0.6371
127	T	A	-0.9268
128	G	A	-1.5319
129	E	A	-2.3566
130	T	A	-1.9905
131	G	A	-1.6512
132	Y	A	-0.9865
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9142
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4151
140	P	A	0.7394
141	V	A	1.7949