Project name: SH3_E121I

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	EA121I
Energy difference between WT (input) and mutated protein (by FoldX)	1.98718 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.102
• Maximal score value: 1.7964
• Average score: -0.8732
• Total score value: -49.774

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5165
86	L	A	0.7880
87	F	A	0.9228
88	V	A	0.4300
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6253
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1020
99	D	A	-2.9304
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2050
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6486
109	F	A	0.0000
110	Q	A	-0.3723
111	I	A	0.2414
112	L	A	0.5527
113	N	A	-0.4978
114	S	A	-0.9401
115	S	A	-1.4772
116	E	A	-2.4319
117	G	A	-2.0377
118	D	A	-2.3387
119	W	A	-0.8799
120	W	A	-0.5667
121	I	A	-0.1721	mutated: EA121I
122	A	A	0.0000
123	R	A	-1.5294
124	S	A	0.0000
125	L	A	0.0499
126	T	A	-0.4770
127	T	A	-0.8245
128	G	A	-1.3652
129	E	A	-2.2469
130	T	A	-1.4766
131	G	A	-1.1728
132	Y	A	-0.4584
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.8024
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7746
141	V	A	1.7964