Project name: SH3_L86R

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA86R
Energy difference between WT (input) and mutated protein (by FoldX)	1.3084 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.065
• Average score: -1.1488
• Total score value: -65.4816

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	-0.6464
86	R	A	-1.6381	mutated: LA86R
87	F	A	-0.3058
88	V	A	-0.1882
89	A	A	0.0000
90	L	A	-0.1197
91	Y	A	-0.5385
92	D	A	-2.5217
93	Y	A	-1.9321
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.6781
105	K	A	-2.3064
106	G	A	-1.2455
107	E	A	-1.3176
108	K	A	-1.3820
109	F	A	0.0000
110	Q	A	-1.5364
111	I	A	-0.6177
112	L	A	0.1213
113	N	A	-0.8980
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0645
121	E	A	-1.1648
122	A	A	0.0000
123	R	A	-2.0834
124	S	A	0.0000
125	L	A	-0.6476
126	T	A	-0.6428
127	T	A	-0.9334
128	G	A	-1.4789
129	E	A	-2.2991
130	T	A	-1.7435
131	G	A	-1.4991
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1511
137	Y	A	-0.1394
138	V	A	0.0000
139	A	A	0.0572
140	P	A	0.0685
141	V	A	1.0650