Project name: SH3_R96I

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	RA96I
Energy difference between WT (input) and mutated protein (by FoldX)	1.21027 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -2.7249
• Maximal score value: 1.7964
• Average score: -0.7862
• Total score value: -44.8128

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9234
88	V	A	0.4306
89	A	A	0.0000
90	L	A	-0.1495
91	Y	A	-0.5709
92	D	A	-2.5523
93	Y	A	-1.4936
94	E	A	-1.7340
95	A	A	-0.7702
96	I	A	0.8365	mutated: RA96I
97	T	A	-0.8348
98	E	A	-2.3523
99	D	A	-2.4799
100	D	A	-1.2964
101	L	A	0.0000
102	S	A	-1.6455
103	F	A	0.0000
104	H	A	-2.7249
105	K	A	-2.4038
106	G	A	-1.4592
107	E	A	-1.3102
108	K	A	-0.6438
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8642
114	S	A	-1.1440
115	S	A	-1.5746
116	E	A	-2.5276
117	G	A	-2.0850
118	D	A	-2.3629
119	W	A	-0.9623
120	W	A	-0.9940
121	E	A	-1.1402
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.0587
126	T	A	-0.4736
127	T	A	-0.8209
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.7192
131	G	A	-1.5433
132	Y	A	-0.8956
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.8666
136	N	A	-1.1254
137	Y	A	-0.1038
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964