Project name: SH3_W120H

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA120H
Energy difference between WT (input) and mutated protein (by FoldX)	3.54418 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8392
• Average score: -0.9775
• Total score value: -55.7202

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5507
86	L	A	0.8586
87	F	A	1.0513
88	V	A	0.4928
89	A	A	0.0000
90	L	A	-0.1358
91	Y	A	-0.5743
92	D	A	-2.5596
93	Y	A	-1.9312
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4057
106	G	A	-1.4432
107	E	A	-1.2889
108	K	A	-0.6014
109	F	A	0.0000
110	Q	A	-0.4976
111	I	A	-0.0866
112	L	A	0.0861
113	N	A	-0.9950
114	S	A	-1.3299
115	S	A	-1.6712
116	E	A	-2.6361
117	G	A	-2.2176
118	D	A	-2.5411
119	W	A	-1.2601
120	H	A	-1.3587	mutated: WA120H
121	E	A	-1.3015
122	A	A	0.0000
123	R	A	-1.7412
124	S	A	0.0000
125	L	A	0.0721
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6903
131	G	A	-1.5454
132	Y	A	-0.9671
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9979
136	N	A	-1.1832
137	Y	A	-0.1099
138	V	A	0.0000
139	A	A	0.4581
140	P	A	0.8298
141	V	A	1.8392