Project name: SH3_L90H

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA90H
Energy difference between WT (input) and mutated protein (by FoldX)	1.85556 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8203
• Average score: -1.0493
• Total score value: -59.8117

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5306
86	L	A	0.8412
87	F	A	0.8259
88	V	A	0.1238
89	A	A	0.0000
90	H	A	-1.4914	mutated: LA90H
91	Y	A	-1.3701
92	D	A	-2.9207
93	Y	A	-2.1400
94	E	A	-2.6650
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.9799
105	K	A	-2.9202
106	G	A	-1.8116
107	E	A	-1.5089
108	K	A	-0.7556
109	F	A	0.0000
110	Q	A	-0.4936
111	I	A	-0.0519
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7014
124	S	A	0.0000
125	L	A	0.1084
126	T	A	-0.4501
127	T	A	-0.8069
128	G	A	-1.3409
129	E	A	-2.2315
130	T	A	-1.6868
131	G	A	-1.4992
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.8675
136	N	A	-1.2997
137	Y	A	-0.5026
138	V	A	-0.1358
139	A	A	0.2134
140	P	A	0.8177
141	V	A	1.8203