Project name: SH3_L112M

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA112M
Energy difference between WT (input) and mutated protein (by FoldX)	1.31564 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1025
• Maximal score value: 1.7964
• Average score: -0.9979
• Total score value: -56.8809

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4744
86	L	A	0.6945
87	F	A	0.8572
88	V	A	0.3858
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5597
93	Y	A	-1.9312
94	E	A	-2.6448
95	A	A	-2.6253
96	R	A	-2.9850
97	T	A	-2.6607
98	E	A	-3.1025
99	D	A	-3.0425
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2033
103	F	A	0.0000
104	H	A	-2.7280
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3382
108	K	A	-0.7404
109	F	A	0.0000
110	Q	A	-0.8176
111	I	A	-0.2602
112	M	A	-0.1283	mutated: LA112M
113	N	A	-1.0167
114	S	A	-1.2247
115	S	A	-1.6232
116	E	A	-2.5604
117	G	A	-2.1343
118	D	A	-2.4445
119	W	A	-1.1027
120	W	A	-1.1211
121	E	A	-1.2554
122	A	A	0.0000
123	R	A	-1.8820
124	S	A	0.0000
125	L	A	-0.1322
126	T	A	-0.5405
127	T	A	-0.8497
128	G	A	-1.4008
129	E	A	-2.2475
130	T	A	-1.7595
131	G	A	-1.5209
132	Y	A	-0.8912
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9178
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7524
141	V	A	1.7964