Project name: SH3_Y93H

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA93H
Energy difference between WT (input) and mutated protein (by FoldX)	3.67969 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1616
• Maximal score value: 1.7964
• Average score: -0.9955
• Total score value: -56.7433

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9258
88	V	A	0.4347
89	A	A	0.0000
90	L	A	-0.1633
91	Y	A	-0.6106
92	D	A	-2.6373
93	H	A	0.0000	mutated: YA93H
94	E	A	-2.7549
95	A	A	-2.7340
96	R	A	-3.0513
97	T	A	-2.7165
98	E	A	-3.1616
99	D	A	-3.1409
100	D	A	-2.2972
101	L	A	0.0000
102	S	A	-2.3053
103	F	A	0.0000
104	H	A	-2.7706
105	K	A	-2.4317
106	G	A	-1.4592
107	E	A	-1.3102
108	K	A	-0.6438
109	F	A	0.0000
110	Q	A	-0.5085
111	I	A	-0.0562
112	L	A	0.1440
113	N	A	-0.9086
114	S	A	-1.2130
115	S	A	-1.6204
116	E	A	-2.5967
117	G	A	-2.1970
118	D	A	-2.5223
119	W	A	-1.2692
120	W	A	-1.1475
121	E	A	-1.2137
122	A	A	0.0000
123	R	A	-1.7149
124	S	A	0.0000
125	L	A	0.0587
126	T	A	-0.4736
127	T	A	-0.8209
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.7213
131	G	A	-1.5834
132	Y	A	-0.9918
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9629
136	N	A	-1.1599
137	Y	A	-0.1421
138	V	A	0.0000
139	A	A	0.4206
140	P	A	0.7757
141	V	A	1.7964