Project name: SH3_G131M

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA131M
Energy difference between WT (input) and mutated protein (by FoldX)	4.65732 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.0434
• Maximal score value: 1.7964
• Average score: -0.9215
• Total score value: -52.5272

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9238
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5611
93	Y	A	-1.9337
94	E	A	-2.6480
95	A	A	-2.5892
96	R	A	-2.9862
97	T	A	-2.6616
98	E	A	-3.0434
99	D	A	-2.9358
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1356
103	F	A	0.0000
104	H	A	-2.7295
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5070
111	I	A	-0.0196
112	L	A	0.1848
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.0610
120	W	A	-0.9969
121	E	A	-1.0335
122	A	A	0.0000
123	R	A	-1.5600
124	S	A	0.0000
125	L	A	0.0954
126	T	A	-0.4385
127	T	A	-0.7491
128	G	A	-1.2129
129	E	A	-1.9386
130	T	A	-1.3997
131	M	A	-1.0922	mutated: GA131M
132	Y	A	-0.6904
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964