Project name: SH3_Y132V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA132V
Energy difference between WT (input) and mutated protein (by FoldX)	1.34341 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1035
• Maximal score value: 1.7964
• Average score: -1.0027
• Total score value: -57.1522

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5183
86	L	A	0.7945
87	F	A	0.9249
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1513
91	Y	A	-0.5752
92	D	A	-2.5604
93	Y	A	-1.9326
94	E	A	-2.6450
95	A	A	-2.6163
96	R	A	-2.9842
97	T	A	-2.6590
98	E	A	-3.0942
99	D	A	-3.1035
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1964
103	F	A	0.0000
104	H	A	-2.7283
105	K	A	-2.4062
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6420
109	F	A	0.0000
110	Q	A	-0.5926
111	I	A	-0.2121
112	L	A	-0.0524
113	N	A	-1.0604
114	S	A	-1.2832
115	S	A	-1.6471
116	E	A	-2.6209
117	G	A	-2.1675
118	D	A	-2.4611
119	W	A	-1.1414
120	W	A	-1.3038
121	E	A	-1.7558
122	A	A	0.0000
123	R	A	-1.8447
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3577
129	E	A	-2.2410
130	T	A	-1.8294
131	G	A	-1.6800
132	V	A	-1.0473	mutated: YA132V
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9638
136	N	A	-1.1369
137	Y	A	-0.1150
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964