Project name: SH3_L112R

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA112R
Energy difference between WT (input) and mutated protein (by FoldX)	1.46576 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1029
• Maximal score value: 1.795
• Average score: -1.1322
• Total score value: -64.5356

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5276
86	L	A	0.8013
87	F	A	0.9247
88	V	A	0.4265
89	A	A	0.0000
90	L	A	-0.1516
91	Y	A	-0.5748
92	D	A	-2.5597
93	Y	A	-1.9312
94	E	A	-2.6448
95	A	A	-2.6256
96	R	A	-2.9850
97	T	A	-2.6607
98	E	A	-3.1029
99	D	A	-3.0535
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2043
103	F	A	0.0000
104	H	A	-2.7285
105	K	A	-2.4065
106	G	A	-1.4592
107	E	A	-1.3306
108	K	A	-0.6624
109	F	A	0.0000
110	Q	A	-1.1112
111	I	A	-1.3259
112	R	A	-2.4713	mutated: LA112R
113	N	A	-2.1848
114	S	A	-1.7888
115	S	A	-1.9163
116	E	A	-2.5716
117	G	A	-2.1427
118	D	A	-2.4537
119	W	A	-1.1221
120	W	A	-1.6138
121	E	A	-2.0795
122	A	A	0.0000
123	R	A	-2.2149
124	S	A	0.0000
125	L	A	-0.0088
126	T	A	-0.5081
127	T	A	-0.8250
128	G	A	-1.3316
129	E	A	-2.2216
130	T	A	-2.0326
131	G	A	-1.8114
132	Y	A	-1.1834
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9317
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4150
140	P	A	0.7811
141	V	A	1.7950