Project name: SH3_H104V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	HA104V
Energy difference between WT (input) and mutated protein (by FoldX)	1.08903 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1042
• Maximal score value: 1.7964
• Average score: -0.7536
• Total score value: -42.956

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9578
88	V	A	0.8149
89	A	A	0.0000
90	L	A	0.3037
91	Y	A	0.1469
92	D	A	-1.2825
93	Y	A	-1.2395
94	E	A	-2.2432
95	A	A	-2.6306
96	R	A	-2.9870
97	T	A	-2.6622
98	E	A	-3.1042
99	D	A	-3.0447
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-1.6600
103	F	A	0.0000
104	V	A	0.1004	mutated: HA104V
105	K	A	-0.9819
106	G	A	-0.6164
107	E	A	0.0000
108	K	A	-0.1982
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.1055
126	T	A	-0.1286
127	T	A	-0.7951
128	G	A	-1.3572
129	E	A	-2.2415
130	T	A	-1.6939
131	G	A	-1.5009
132	Y	A	-0.8689
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964