Project name: SH3_T126V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	TA126V
Energy difference between WT (input) and mutated protein (by FoldX)	-0.639249 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1042
• Maximal score value: 1.7984
• Average score: -0.853
• Total score value: -48.6222

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5161
86	L	A	0.7947
87	F	A	0.9323
88	V	A	0.4473
89	A	A	0.0000
90	L	A	-0.1449
91	Y	A	-0.5710
92	D	A	-2.5579
93	Y	A	-1.9353
94	E	A	-2.6500
95	A	A	-2.6306
96	R	A	-2.9870
97	T	A	-2.6622
98	E	A	-3.1042
99	D	A	-3.0446
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2177
103	F	A	0.0000
104	H	A	-2.5259
105	K	A	-2.3942
106	G	A	-1.2485
107	E	A	-0.8188
108	K	A	-0.2061
109	F	A	0.0000
110	Q	A	-0.5065
111	I	A	-0.0556
112	L	A	0.1430
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1529
122	A	A	0.0000
123	R	A	-1.4526
124	S	A	0.0000
125	L	A	0.9871
126	V	A	1.3816	mutated: TA126V
127	T	A	0.0739
128	G	A	-0.8614
129	E	A	-1.9441
130	T	A	-1.6937
131	G	A	-1.5008
132	Y	A	-0.8688
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4174
140	P	A	0.7769
141	V	A	1.7984