Project name: SH3_V88E

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA88E
Energy difference between WT (input) and mutated protein (by FoldX)	1.57971 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.4691
• Average score: -1.0608
• Total score value: -60.4632

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4012
86	L	A	0.5296
87	F	A	0.3509
88	E	A	-0.7287	mutated: VA88E
89	A	A	0.0000
90	L	A	-0.4961
91	Y	A	-0.7440
92	D	A	-2.5716
93	Y	A	-1.9385
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.8673
105	K	A	-2.6416
106	G	A	-1.8261
107	E	A	-1.6763
108	K	A	-1.1472
109	F	A	0.0000
110	Q	A	-0.6578
111	I	A	-0.0573
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7161
124	S	A	0.0000
125	L	A	-0.0669
126	T	A	-0.4739
127	T	A	-0.8211
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.4992
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1586
137	Y	A	-0.3189
138	V	A	0.0000
139	A	A	0.0337
140	P	A	0.4770
141	V	A	1.4691