Project name: SH3_L86D

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA86D
Energy difference between WT (input) and mutated protein (by FoldX)	1.77524 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.4869
• Average score: -1.0357
• Total score value: -59.0365

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	-0.0944
86	D	A	-0.3850	mutated: LA86D
87	F	A	0.3179
88	V	A	0.1519
89	A	A	0.0000
90	L	A	-0.1126
91	Y	A	-0.5401
92	D	A	-2.5221
93	Y	A	-1.9321
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0436
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2061
103	F	A	0.0000
104	H	A	-2.6681
105	K	A	-2.2959
106	G	A	-1.2259
107	E	A	-1.1507
108	K	A	-0.8642
109	F	A	0.0000
110	Q	A	-1.3329
111	I	A	-0.4880
112	L	A	0.0886
113	N	A	-0.9142
114	S	A	-1.1623
115	S	A	-1.5884
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0903
121	E	A	-1.1936
122	A	A	0.0000
123	R	A	-1.8811
124	S	A	0.0000
125	L	A	0.0580
126	T	A	-0.3206
127	T	A	-0.7472
128	G	A	-1.3404
129	E	A	-2.1947
130	T	A	-1.7074
131	G	A	-1.4836
132	Y	A	-0.8544
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9195
136	N	A	-1.1511
137	Y	A	-0.1408
138	V	A	0.0000
139	A	A	0.2622
140	P	A	0.4103
141	V	A	1.4869