Project name: SH3_L90V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA90V
Energy difference between WT (input) and mutated protein (by FoldX)	1.24496 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8137
• Average score: -0.9528
• Total score value: -54.3092

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5228
86	L	A	0.8081
87	F	A	0.9559
88	V	A	0.4923
89	A	A	0.0000
90	V	A	-0.1082	mutated: LA90V
91	Y	A	-0.5552
92	D	A	-2.5524
93	Y	A	-1.9241
94	E	A	-2.6426
95	A	A	-2.6246
96	R	A	-2.9846
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2042
103	F	A	0.0000
104	H	A	-2.7153
105	K	A	-2.3852
106	G	A	-1.4320
107	E	A	-1.2826
108	K	A	-0.6060
109	F	A	0.0000
110	Q	A	-0.5004
111	I	A	-0.0555
112	L	A	0.1429
113	N	A	-0.8883
114	S	A	-1.1799
115	S	A	-1.5966
116	E	A	-2.5603
117	G	A	-2.1347
118	D	A	-2.4456
119	W	A	-1.1062
120	W	A	-1.0569
121	E	A	-1.1536
122	A	A	0.0000
123	R	A	-1.7137
124	S	A	0.0000
125	L	A	0.0683
126	T	A	-0.4703
127	T	A	-0.8194
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.4995
132	Y	A	-0.8681
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9232
136	N	A	-1.1496
137	Y	A	-0.1144
138	V	A	0.0000
139	A	A	0.4442
140	P	A	0.7928
141	V	A	1.8137