Project name: SH3_L125D

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA125D
Energy difference between WT (input) and mutated protein (by FoldX)	1.97437 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8247
• Average score: -1.1589
• Total score value: -66.0583

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5231
86	L	A	0.4472
87	F	A	0.6171
88	V	A	0.1071
89	A	A	0.0000
90	L	A	-0.1521
91	Y	A	-0.5750
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7289
105	K	A	-2.4069
106	G	A	-1.4598
107	E	A	-1.9140
108	K	A	-1.6075
109	F	A	0.0000
110	Q	A	-1.2988
111	I	A	-0.1423
112	L	A	0.0871
113	N	A	-0.9104
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0794
121	E	A	-1.1870
122	A	A	0.0000
123	R	A	-2.5404
124	S	A	0.0000
125	D	A	-2.8855	mutated: LA125D
126	T	A	-1.9232
127	T	A	-1.6676
128	G	A	-2.2737
129	E	A	-2.7176
130	T	A	-2.0525
131	G	A	-1.5075
132	Y	A	-0.8724
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1246
138	V	A	0.0000
139	A	A	0.4289
140	P	A	0.7819
141	V	A	1.8247