Project name: SH3_E121C

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	EA121C
Energy difference between WT (input) and mutated protein (by FoldX)	-0.0432785 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.0944
• Maximal score value: 1.7964
• Average score: -0.9086
• Total score value: -51.7899

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5183
86	L	A	0.7945
87	F	A	0.9250
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6167
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.0944
99	D	A	-2.9512
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1971
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6420
109	F	A	0.0000
110	Q	A	-0.4276
111	I	A	0.1020
112	L	A	0.3317
113	N	A	-0.7955
114	S	A	-1.0981
115	S	A	-1.5695
116	E	A	-2.5145
117	G	A	-2.0927
118	D	A	-2.3831
119	W	A	-0.9560
120	W	A	-0.7615
121	C	A	-0.5361	mutated: EA121C
122	A	A	0.0000
123	R	A	-1.5650
124	S	A	0.0000
125	L	A	0.0589
126	T	A	-0.4730
127	T	A	-0.8210
128	G	A	-1.3579
129	E	A	-2.2414
130	T	A	-1.5326
131	G	A	-1.2505
132	Y	A	-0.5478
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.8317
136	N	A	-1.1500
137	Y	A	-0.1241
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964