Project name: SH3_N113C

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	NA113C
Energy difference between WT (input) and mutated protein (by FoldX)	1.106 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1026
• Maximal score value: 1.7964
• Average score: -0.9045
• Total score value: -51.5576

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9238
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6256
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1026
99	D	A	-3.0330
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2056
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.4457
111	I	A	0.1164
112	L	A	0.4014
113	C	A	-0.1266	mutated: NA113C
114	S	A	-0.8055
115	S	A	-1.3645
116	E	A	-2.3584
117	G	A	-2.0146
118	D	A	-2.3475
119	W	A	-0.9643
120	W	A	-0.7853
121	E	A	-0.8256
122	A	A	0.0000
123	R	A	-1.7307
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6951
131	G	A	-1.4886
132	Y	A	-0.7435
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9092
136	N	A	-1.1500
137	Y	A	-0.1241
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964