Project name: sabu Varghese [mutate: WA25A, FA22A]

Chain sequence(s)	A: HSQGTFTSDYSKYLDSRRAQDFVQWLMNT
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA25A, FA22A
Energy difference between WT (input) and mutated protein (by FoldX)	0.263781 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -4.1285
• Maximal score value: 1.4991
• Average score: -1.2547
• Total score value: -36.3859

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	H	A	-1.6165
2	S	A	-1.3983
3	Q	A	-1.4914
4	G	A	-0.8818
5	T	A	-0.0518
6	F	A	1.3502
7	T	A	0.2347
8	S	A	-0.3362
9	D	A	-0.5532
10	Y	A	0.2704
11	S	A	-0.6390
12	K	A	-2.1082
13	Y	A	-1.1309
14	L	A	-1.3588
15	D	A	-3.1590
16	S	A	-2.9946
17	R	A	-3.9714
18	R	A	-4.1285
19	A	A	-3.0267
20	Q	A	-3.4794
21	D	A	-3.7748
22	A	A	-1.6220	mutated: FA22A
23	V	A	-0.7381
24	Q	A	-1.2924
25	A	A	0.0568	mutated: WA25A
26	L	A	1.4991
27	M	A	0.9876
28	N	A	-0.7187
29	T	A	-0.3130