Project name: SH3_Y137W

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA137W
Energy difference between WT (input) and mutated protein (by FoldX)	2.53077 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7964
• Average score: -0.9553
• Total score value: -54.453

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9238
88	V	A	0.4314
89	A	A	0.0000
90	L	A	-0.1354
91	Y	A	-0.5367
92	D	A	-2.4810
93	Y	A	-1.9116
94	E	A	-2.6367
95	A	A	-2.6228
96	R	A	-2.9880
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1975
103	F	A	0.0000
104	H	A	-2.6933
105	K	A	-2.3746
106	G	A	-1.4442
107	E	A	-1.2955
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0569
112	L	A	0.1429
113	N	A	-0.8883
114	S	A	-1.1800
115	S	A	-1.5966
116	E	A	-2.5603
117	G	A	-2.1348
118	D	A	-2.4458
119	W	A	-1.1054
120	W	A	-1.0570
121	E	A	-1.1536
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.4995
132	Y	A	-0.8682
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9217
136	N	A	-1.1508
137	W	A	-0.1147	mutated: YA137W
138	V	A	0.0000
139	A	A	0.4159
140	P	A	0.7757
141	V	A	1.7964