Project name: SH3_W119P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA119P
Energy difference between WT (input) and mutated protein (by FoldX)	2.56898 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1005
• Maximal score value: 1.7964
• Average score: -1.0249
• Total score value: -58.4215

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9240
88	V	A	0.4316
89	A	A	0.0000
90	L	A	-0.1470
91	Y	A	-0.5669
92	D	A	-2.5455
93	Y	A	-1.9071
94	E	A	-2.6312
95	A	A	-2.6199
96	R	A	-2.9819
97	T	A	-2.6608
98	E	A	-3.1005
99	D	A	-3.0383
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1945
103	F	A	0.0000
104	H	A	-2.7210
105	K	A	-2.4006
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5087
111	I	A	-0.0539
112	L	A	0.1464
113	N	A	-0.9824
114	S	A	-1.3227
115	S	A	-1.6809
116	E	A	-2.6868
117	G	A	-2.3243
118	D	A	-2.7922
119	P	A	-1.8258	mutated: WA119P
120	W	A	-1.4122
121	E	A	-1.3156
122	A	A	0.0000
123	R	A	-1.7128
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.6888
131	G	A	-1.5756
132	Y	A	-1.0740
133	I	A	0.0000
134	P	A	-0.8687
135	S	A	-1.1658
136	N	A	-1.2697
137	Y	A	-0.2057
138	V	A	0.0000
139	A	A	0.4161
140	P	A	0.7757
141	V	A	1.7964