Project name: SH3_S135V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	SA135V
Energy difference between WT (input) and mutated protein (by FoldX)	2.03287 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7944
• Average score: -0.9429
• Total score value: -53.7434

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5145
86	L	A	0.7886
87	F	A	0.9522
88	V	A	0.4287
89	A	A	0.0000
90	L	A	-0.1513
91	Y	A	-0.5743
92	D	A	-2.5596
93	Y	A	-1.9312
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4057
106	G	A	-1.4588
107	E	A	-1.3087
108	K	A	-0.6453
109	F	A	0.0000
110	Q	A	-0.5163
111	I	A	-0.0367
112	L	A	0.1379
113	N	A	-0.8900
114	S	A	-1.1288
115	S	A	-1.5909
116	E	A	-2.5553
117	G	A	-2.0862
118	D	A	-2.3529
119	W	A	-1.0198
120	W	A	-0.9908
121	E	A	-1.1266
122	A	A	0.0000
123	R	A	-1.7194
124	S	A	0.0000
125	L	A	0.0585
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6936
131	G	A	-1.5040
132	Y	A	-0.8360
133	I	A	0.0000
134	P	A	0.0000
135	V	A	-0.6379	mutated: SA135V
136	N	A	-1.0141
137	Y	A	-0.0311
138	V	A	0.0000
139	A	A	0.4624
140	P	A	0.7731
141	V	A	1.7944