Project name: SH3_Y93T

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA93T
Energy difference between WT (input) and mutated protein (by FoldX)	5.04551 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1571
• Maximal score value: 1.7964
• Average score: -1.0245
• Total score value: -58.3951

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9279
88	V	A	0.4365
89	A	A	0.0000
90	L	A	-0.1607
91	Y	A	-0.6102
92	D	A	-2.6404
93	T	A	-2.1203	mutated: YA93T
94	E	A	-2.7573
95	A	A	-2.7313
96	R	A	-3.0483
97	T	A	-2.7117
98	E	A	-3.1571
99	D	A	-3.1338
100	D	A	-2.2731
101	L	A	0.0000
102	S	A	-2.3053
103	F	A	0.0000
104	H	A	-2.7732
105	K	A	-2.4319
106	G	A	-1.4592
107	E	A	-1.3102
108	K	A	-0.6438
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0573
112	L	A	0.1425
113	N	A	-0.8969
114	S	A	-1.1923
115	S	A	-1.6039
116	E	A	-2.5713
117	G	A	-2.1512
118	D	A	-2.4737
119	W	A	-1.1847
120	W	A	-1.1078
121	E	A	-1.1948
122	A	A	0.0000
123	R	A	-1.7161
124	S	A	0.0000
125	L	A	0.0587
126	T	A	-0.4736
127	T	A	-0.8209
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.7199
131	G	A	-1.5716
132	Y	A	-0.9672
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9276
136	N	A	-1.1405
137	Y	A	-0.1218
138	V	A	0.0000
139	A	A	0.4241
140	P	A	0.7757
141	V	A	1.7964