Project name: SH3_W119D

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA119D
Energy difference between WT (input) and mutated protein (by FoldX)	2.74847 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.5263
• Maximal score value: 1.7964
• Average score: -1.1454
• Total score value: -65.2891

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9240
88	V	A	0.4316
89	A	A	0.0000
90	L	A	-0.1470
91	Y	A	-0.5669
92	D	A	-2.5452
93	Y	A	-1.9063
94	E	A	-2.6304
95	A	A	-2.6190
96	R	A	-2.9815
97	T	A	-2.6606
98	E	A	-3.1006
99	D	A	-3.0387
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1932
103	F	A	0.0000
104	H	A	-2.7207
105	K	A	-2.4006
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5085
111	I	A	-0.0536
112	L	A	0.1471
113	N	A	-1.1945
114	S	A	-1.6417
115	S	A	-1.8796
116	E	A	-2.9849
117	G	A	-2.7826
118	D	A	-3.5263
119	D	A	-3.3086	mutated: WA119D
120	W	A	-2.1547
121	E	A	-1.6651
122	A	A	0.0000
123	R	A	-1.7132
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2400
130	T	A	-1.6891
131	G	A	-1.7522
132	Y	A	-1.5206
133	I	A	0.0000
134	P	A	-1.4651
135	S	A	-1.6465
136	N	A	-1.4908
137	Y	A	-0.3621
138	V	A	0.0000
139	A	A	0.4162
140	P	A	0.7757
141	V	A	1.7964