Project name: SH3_Y93R

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA93R
Energy difference between WT (input) and mutated protein (by FoldX)	3.83633 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.095
• Maximal score value: 1.7964
• Average score: -1.0048
• Total score value: -57.2723

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9216
88	V	A	0.4296
89	A	A	0.0000
90	L	A	-0.2181
91	Y	A	-0.7083
92	D	A	-2.8110
93	R	A	-2.4425	mutated: YA93R
94	E	A	-2.8919
95	A	A	-2.7493
96	R	A	-3.0355
97	T	A	-2.6444
98	E	A	-3.0950
99	D	A	-3.0384
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.3699
103	F	A	0.0000
104	H	A	-2.8555
105	K	A	-2.4953
106	G	A	-1.4570
107	E	A	-1.3055
108	K	A	-0.6415
109	F	A	0.0000
110	Q	A	-0.5085
111	I	A	-0.0563
112	L	A	0.1439
113	N	A	-0.9108
114	S	A	-1.2160
115	S	A	-1.6219
116	E	A	-2.5989
117	G	A	-2.1999
118	D	A	-2.5294
119	W	A	-1.2612
120	W	A	-1.1322
121	E	A	-1.1891
122	A	A	0.0000
123	R	A	-1.7151
124	S	A	0.0000
125	L	A	0.0596
126	T	A	-0.4724
127	T	A	-0.8204
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.6930
131	G	A	-1.5168
132	Y	A	-0.9130
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9818
136	N	A	-1.1830
137	Y	A	-0.2449
138	V	A	0.0000
139	A	A	0.4115
140	P	A	0.7757
141	V	A	1.7964